LPA经PI3K/Akt信号转导通路抑制顺铂诱导卵巢癌细胞凋亡

 目的 研究PI3K/AKT信号转导通路对LPA保护顺铂诱导的卵巢癌SKOV3细胞凋亡作用的影响。 方法 MTT法检测LPA和LY294002对DDP作用后的SKOV3细胞增殖活性的影响；Hoechst33258荧光染色 观察凋亡细胞；FCM分析细胞凋 亡率；凝胶电泳观察凋亡细胞的DNA“梯状”条带；Western blot检测LPA、LY294002对磷酸 化Akt蛋白表达的影响。 结果 LPA+LY294002+DDP组对SKOV3细胞增殖的抑制作用、凋亡小体的产生及细胞凋亡率高于 LPA+DDP组(P<0.05)，而与 LY294002+DDP组差异无统计学意义， DNA片段凝胶电泳示LPA作用后不产生明显的凋亡片段， LPA和LY294002同时作用可出现DNA断裂梯形条带。Western blot结果示LPA作用后磷酸化Akt 蛋白表达升高，而LY294002作用后，磷酸化Akt蛋白表达明显下降。 结论 LPA通过激活PI3K/Akt信号转导通路抑制顺铂诱导的卵巢癌细胞的凋亡。

LPA Inhibits Apoptosis Induced by Cisplatin in Ovarian Carcinoma Cells via PI3K/Akt Signaling Pathway

Objective This study aims to investigate the relationship between PI3K/AKt signaling pathway and the protection provided by LPA for cisplatin-induced apoptosis in ovarian carcinoma cells. Methods MTT assay was used to assess the proliferative activity of SKOV3 cells treated by DDP with or without LPA and LY294002. Hochest33258 was used to observe the apoptotic cells by fluorescence staining. FCM was used to detect the apoptosis of SKOV3 cells treated by DDP with LPA and LY294002. The specific DNA ladder pat...