结肠癌中VEGF-D和uPA的表达与侵袭转移的关系

目的探讨血管内皮生长因子-D（endothelial growthfactor—D，VEGF—D）和尿激酶型纤维蛋白酶原激活剂（urokinase—type plasminogen activator，uPA）在结肠癌中的表达特点及临床意义。方法选取30例结肠癌组织及同一段肠管距离肿瘤组织5cm以上的正常组织，分别应用免疫组化S-P法、Westernblot方法检测VEGF—D和uPA在结肠癌组织和正常组织中的表达特点，分析它们与临床病理分期之间的关系。结果VEGF—D和uPA在结肠癌组织和正常组织中均有表达，主要表达于细胞质，胞核也有表达，并且在肿瘤组织中的表达明显高于正常组织（P〈0．01）。VEGF—D或uPA的表达活性在DukesC＋D期明显高于A＋B期（P〈0．01），在有淋巴结转移组和远处转移组的表达也高于相应无转移组（P〈0．05、P〈0．01），随着肿瘤组织分化程度的增高活性依次降低（P〈0．05）。结论VEGF—D和uPA在结肠癌组织中的高表达可能在结肠癌的发生发展过程中扮演重要角色。

Relationship of VEGF-D and uPA with invasion and metastasis of human colon carcinoma

Objective To explore the expressions of endothelial growth factor-D (VEGF-D) and urokinase-type plasminogen activator (uPA) in colon carcinoma and to reveal their correlation with the tumor invasion and metastasis. Methods The expressions of VEGF-D and uPA were detected by immunohistochemistry and Western blotting in 30 specimens of colon carcinoma and 30 specimens of normal neighbouring colon tissue 5 cm away from the lesions. Results The expressions of VEGF-D and uPA mostly located in endochylema, less in...