苯那普利对糖尿病大鼠肾脏结缔组织生长因子mRNA表达的影响

目的：观察苯那普利对糖尿病（DM）大鼠肾脏结缔组织生长因子（CTGF）基因表达和肾脏病 变的影响。 方法：将实验动物分为正常对照组（CON）、糖尿病组（DM）和苯那普利治疗组（DM+B）。 苯那普利处理12周后检测血清肌酐、尿素氮水平、尿白蛋白排泄率（UAER）和肾皮质血管紧张素Ⅱ（AngⅡ）含量，RT-PCR检测肾皮质CTGF mRNA表达，免疫组化检测肾纤维连接蛋白（FN）和Ⅳ型胶原（Col Ⅳ）蛋白表达水平。 结果：实验终止时，DM组UAER（12.92±3.56）mg·24 h^-1明显高于CON组（2.74±1.09）mg·24 h^-1（P<0.01），DM+B组大鼠UAER（5.69±2.74）mg·24 h^-1明显低于DM组（P<0.01）。与CON组相比，DM组血清肌酐和尿素氮水平均明显增高（P<0.01）；苯那普利治疗使其水平明显降低（P<0.01）。DM大鼠肾皮质CTGF mRNA表达水平约为CON组的2.65倍（P<0.01），在DM+B组其表达水平减少23.68%（P<0.05）。DM大鼠肾小球FN和Col Ⅳ蛋白表达均较CON组明显上调（均为P<0.01），苯那普利治疗后其表达水平明显降低（P<0.01）。 结论：苯那普利能降低DM大鼠肾脏CTGF基因表达，并能明显减轻肾病变。

Effect of benazepril on connective tissue growth factor mRNA expression in kidney of diabetic rats

Objective To investigate the effect of benazepril on expression of connective tissue growth factor(CTGF)gene in kidney of diabetic rats and renal lesion.Methods Forty Wistar rats were randomly divided into control group(CON),diabetic group(DM) and diabetic rats treated with benazepril(DM+B) group.After benazepril was orally administered(15 mg·kg~(-1)·d~(-1))to the diabetic rats for 12 weeks,serum creatinine(Scr) and blood urea nitrogen(BUN) levels,urinary protein excretion(UAER)and angiotensin Ⅱ(AngⅡ) contents in renal cortex were determined.CTGF mRNA expression in the renal cortex was detected by RT-PCR.Immunohistochemstry was used to examine the expressions of fibronectin(FN) and type Ⅳ collagen(Col Ⅳ) in glomeruli.Results At the end of the experiment,UAER in DM group(12.92±3.56 mg·24 h~(-1)) was significantly higher than that in CON group(2.74±1.09 mg·24 h~(-1))(P<0.01).The UAER in DM+B group(5.69±2.74 mg·24 h~(-1)) was markedly lower than that in DM group(P<0.01).Diabetic rats showed an increase tendency in Scr(P<0.01) and BUN(P<0.01) contents compared with CON group,which were significantly reduced by benazepril treatment(P<0.01).CTGF mRNA expression in the renal cortex of DM group was (2.65 times) that of CON group,and the expression of DM+B group was lower by 23.68% compared with that of DM group.The glomerular protein expressions of FN and Col IV were markedly up-regulated in DM group as compared with those in CON group(all P<0.01).Benazepril obviously decreased the expressions of FN and Col Ⅳ.Conclusion Benazepril reduces the increased CTGF mRNA expression in kidney of diabetic rats and ameliorates the renal lesions.