阿那日五味散挥发油不同提取工艺比较及包合工艺研究

目的 研究阿那日五味散中挥发油组分的不同提取方法的最佳工艺以及β-环糊精包合挥发油的最佳工艺.方法 采用水蒸汽蒸馏法和二氧化碳超临界萃取法提取挥发油;采用饱和水溶液法,运用正交试验,以包合物收得率为评价指标筛选最佳包合工艺.结果 阿那日五味散水蒸汽蒸馏法提取挥发油的最佳工艺为加入药材8倍量的水提取4h;二氧化碳超临界萃取挥发油的最佳工艺为A3B3C3D2,即提取罐的压力为25 MPa,提取罐的温度为45℃,提取2.0 h,分离罐Ⅱ的温度为35℃;水蒸汽蒸馏法提取挥发油的最佳包合工艺为A1B1C3,即：挥发油与β-环糊精比例1∶3,包合温度30℃,水浴搅拌2.0 h;二氧化碳超临界萃取的挥发油的最佳包合工艺条件为A1B2C3D2,即：挥发油与β-环糊精用量比例为1∶3,包合温度为35℃,包合时间为2.0 h,挥发油与无水乙醇比例为1∶1.结论 二氧化碳超临界萃取挥发油的量是水蒸汽蒸馏法的2倍多,能够极大地提升提取效率,使用β-环糊精包合挥发油可提高挥发油的稳定性,该方法简便易行,适用于大规模生产.

Study on the different extraction technology and inclusion technology of volatile oil of Anari Wuwei Powder

Objective To choose the best technology of different extractions and the best β-cycledextrin inclusion technology of olatile oil.Methods The volatile oil was extracted by steam distillation and supercritical carbon dioxide extraction; the study was carried out with orthogonal design; the inclusion technology was determined by the ratio of inclusion compound.Results The best technology of steam distillation to extract the volatile oil was：adding 8 times amount of water extraction for 3.5 hours; the best technology of supercritical carbon dioxide extraction of volatile oil was A3B3C3D2,that were：25 mPa,45℃,extracted 2.0 hours,the temperature of separate 2 was 35℃; the best inclusion process of the volatile oil extracted by steam distillation was A1B1C3,that were：the ratio of volatile oil and β-CD was 1∶5,the inclusion temperature was 30℃,the inclusion time was 2.0 hours; the best inclusion process of volatile oil extracted by supercritical carbon dioxide extraction was A1B2C3D2,that were：the ratio of volatile oil and β-CD was 1∶3,the inclusion temperature was 35℃,the inclusion time was 2.0 hours,the volatile oil：anhydrous ethanol was 1∶1.Conclusion The volatile oil content extracted by supercritical carbon dioxide extraction is higher than the steam distillation method for more than 2 times,this process can improve the stability of the inclusion,the method is convenient and can be applied to the large-scale production.