液相色谱-串联质谱法分析10种水溶性维生素

目的：建立水溶性维生素的液相色谱-质谱检测方法。方法：直接用乙腈：水：甲酸（15：85：0.1,v/v/v）为流动相进行反相液相色谱分离,用电喷雾正离子源进行离子化,选择反应监测方式（SRM）对这10种水溶性维生素的母离子及子离子进行监测,三级四极质谱测定。结果：该方法抗干扰能力强,方法重现性好,RSD为0.6%～2.4%,回收率97.9%～104.7%,相关系数0.9967～0.9998,检出限0.1～0.8 ng/L。结论：该方法快速、准确、灵敏,适合测定各种样品中的水溶性维生素。

Analysis of 10 water soluble vitamins by liquid chromatography -tandem mass spectrometry

Objective:To establish a sensitive method for determination of water soluble vitamins by liquid chromatography-tandem mass spectrometry.Methods:The extract was separated by reverse phase liquid chromatography using acetonitrile:water:formic acid(15:85:0.1,v/v/v) as the elution solvents at 0.2 ml/min drip rate,confirmation and quantification by mass spectrum in the select reaction monitoring(SRM) mode via positive electrospray ionization(+ESI).Results:The samples were very simple,the method had strong anti-jamming ability,the calibration curves of the water soluble vitamins were excellent with correlation coefficients between 0.9967 and 0.9998,and RSD between 0.6% and 2.4%,the recoveries between 97.9% and 104.7%,the detection limits 0.1~0.8 ng/L.Conclusion:The results established method is quite suitable for identification and quantization of amino acids.