GAD67-GFP转基因小鼠三叉神经脊束核吻侧亚核和极间亚核内GABA和甘氨酸共存神经元的观察

本文以GAD67-GFP基因敲入小鼠为研究工具,利用免疫荧光组织化学双重染色技术观察了小鼠三叉神经脊束核(spi-nal trigeminalnucleus,V)的吻侧亚核(Vo)和极间亚核(Vi)神经元内谷氨酸脱羧酶67GAD67,γ-氨基丁酸(GABA)能神经元的标识物质]和甘氨酸(glycine,Gly)的定位分布和共存情况。结果显示,在Vo和Vi内均有比较密集分布的GAD67和Gly阳性神经元。此外,在Vo和Vi内也可见一些GAD67阳性神经元同时呈Gly阳性反应,即存在GAD67/Gly双标神经元。结果表明,Vo和Vi内有GABA和Gly共存神经元,它们可能在初级传入信息的传递和调制中发挥重要作用。

COEXISTENCE OF GABA AND GLYCINE IN THE NEURONS OF THE ORAL AND INTERPOLAR SUBNUCLEI OF THE SPINAL TRIGEMINAL NUCLEUS IN THE GAID67-GFP TRANSGENIC MOUSE

The present study was designed to observe the distribution and coexistence of glutamic acid decarboxylase 67 GAD67, the marker of the γ-aminobutyric acid (GABA)-ergic neuron] and glycine (Gly) in neurons of the oral subnucleus (Vo) and interpolaris subnucleus (Vi) of the spinal trigeminal nucleus (V) in the GAD67-green fluorescent protein (GFP) transgenic mouse. GAD67-GFP knock-in mouse and immunofluorescence histochemical double-staining technique were used. The results showed that GAD67 immunoreactive (GAD67-IR) and Gly-IR neurons were quite densely distributed in the Vo and Vi. Some of the GAD67-IR neurons also showed Gly-immunoreactivities, i.e. there were GAD67/Gly double-labeled neurons, in the Vo and Vi. The present results indicate that there are GABA and glycine co-existed neurons in the Vo and Vi. They might exert important effects on the transmission and regulation of the primary sensory information.