| 胰岛素样生长因子-Ⅰ和骨形态发生蛋白-2对人牙周膜细胞增殖的作用 |
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| 引用本文： | 刘宏,吴织芬,王勤涛. 胰岛素样生长因子-Ⅰ和骨形态发生蛋白-2对人牙周膜细胞增殖的作用[J]. 牙体牙髓牙周病学杂志, 1999, 9(1) |
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| 作者姓名： | 刘宏 吴织芬 王勤涛 |
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| 作者单位： | 第四军医大学口腔医学院 |
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| 摘 要： | 目的：重组人胰岛素样生长因子－Ｉ（ｒｈＩＧＦ－Ｉ）、重组人骨形态发生蛋白－２（ｒｈＢＭＰ－２）分别或联合应用对人牙周膜（ＰＤＬ）细胞增殖的影响。方法：采用组织块法体外培养人ＰＤＬ细胞，ＭＴＴ法测定ＰＤＬ细胞在不同生长因子刺激下的增殖情况。结果：ｒｈＩＧＦ－Ｉ、ｒｈＢＭＰ－２都可促进人ＰＤＬ细胞的增殖，这种促增殖作用呈一定的浓度依赖性，ｒｈＩＧＦ－Ｉ与ｒｈＢＭＰ－２联合应用对人ＰＤＬ细胞的增殖有协同作用，且与单独应用相比相差显著。结论：ｒｈＩＧＦ－Ｉ、ｒｈＢＭＰ－２可望作为牙周再生的生物活性介质，ｒｈＩＧＦ－Ｉ与ｒｈＢＭＰ－２联合应用对ＰＤＬ细胞的促增殖作用更强。
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| 关 键 词： | 牙周膜细胞；细胞培养；胰岛素样生长因子；骨形态发生蛋白 |
Effect of recombinant human Insulin-like growth factor-I and recombinant human bone morphogenetic protein-2 on proliferation of human periodontal ligament cells |
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| Liu Hong,Wu Zhifen,Wang Qintao School of Stomatology,The Fourth Military Medical University,Xi'an. Effect of recombinant human Insulin-like growth factor-I and recombinant human bone morphogenetic protein-2 on proliferation of human periodontal ligament cells[J]. Chinese Journal of Conservative Dentistry, 1999, 9(1) |
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| Authors: | Liu Hong Wu Zhifen Wang Qintao School of Stomatology The Fourth Military Medical University Xi'an |
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| Affiliation: | Liu Hong,Wu Zhifen,Wang Qintao School of Stomatology,The Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | Aim: To study the effect of recombinant human Insulin-like growth factor-I and recombinant human bone morphogenetic protein-2 on human periodontal ligament cells proliferation. Methods: Human periodontal ligament cells were cultured by tissue explant and its proliferation were mesured by MTT colorimetric assay. Results: rhIGF-I or rhBMP-2 can stimulate human PDL cells proliferation in dose-dependent manner. The co-ordinate use of rhIGF-I and rhBMP-2 has synergetic effect on PDL cells proliferation. Conclusions: rhIGF-I and rhBMP-2 can be used as bioactive mediator on periodontal regeneration. |
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| Keywords: | periodontal ligament cells cell culture recombinant human insulin-like growth factor-I recombinant human bone morphogenetic protein-2 |
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