| 神经上皮细胞转化基因1对细胞辐射敏感性的影响及其机制探讨 |
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| 引用本文： | 徐畅,王彦,杜利清,王芹,刘强.神经上皮细胞转化基因1对细胞辐射敏感性的影响及其机制探讨[J].中华放射医学与防护杂志,2015,35(1):49-52. |
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| 作者姓名： | 徐畅 王彦 杜利清 王芹 刘强 |
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| 作者单位： | 北京协和医学院中国医学科学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津,300192 |
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| 基金项目： | 国家自然科学基金(31200634, 31170804);天津市自然科学基金(12JCYBJC32900) |
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| 摘 要： | 目的 探讨神经上皮细胞转化基因1(Net1)对细胞辐射敏感性的影响及相关的分子作用机制.方法 运用实时荧光定量PCR检测辐射后细胞中Net1基因表达水平的变化;采用RNAi干扰技术抑制细胞中Net1的表达,用克隆形成率分析细胞的辐射敏感性;利用免疫共沉淀技术发现Net1的结合蛋白.结果 电离辐射损伤后,细胞中的Net1 mRNA水平显著上升(t=-10.52,P＜0.05);与对照组相比,siRNA沉默细胞中的Net1表达后明显增加了细胞的辐射敏感性(t=15.31、11.65,P＜0.05);无论在正常状态下还是在细胞受到辐照后,Net1都能与非同源末端连接修复蛋白Ku70、Ku80和DNA-PKcs结合.结论 Net1对细胞的辐射防护作用可能是通过与非同源末端连接修复蛋白相互作用来调控辐射损伤修复实现的.
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| 关 键 词： | 神经上皮细胞转化基因1 辐射敏感性 DNA双链断裂 非同源末端连接 |
| 收稿时间： | 2014/10/14 0:00:00 |
Effect and mechanism of neuroepithelial cell transforming gene 1 on cellular radiosensitivity |
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| Xu Chang,Wang Yan,Du Liqing,Wang Qin and Liu Qiang.Effect and mechanism of neuroepithelial cell transforming gene 1 on cellular radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2015,35(1):49-52. |
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| Authors: | Xu Chang Wang Yan Du Liqing Wang Qin and Liu Qiang |
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| Institution: | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China;Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China;Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China;Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China;Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China |
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| Abstract: | |
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| Keywords: | Net1 Radiosensitivity DNA double-strand break Non-homologous end joining |
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