| 青霉素酰化酶的分离和纯化 |
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| 引用本文： | 武金霞,张贺迎. 青霉素酰化酶的分离和纯化[J]. 中国医药工业杂志, 2002, 33(4): 161-163 |
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| 作者姓名： | 武金霞 张贺迎 |
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| 作者单位： | 河北大学生命科学学院,河北保定,071002 |
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| 基金项目： | 河北省教委资助项目 (973 0 1) |
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| 摘 要： | 采用渗透法冲击法提取大肠杆菌E.coli108青霉素酰化酶，得到了高比活的粗酶液，收率为93．3％，经硫酸铵分级沉淀、DEAE－Cellulose－52离子交换柱层析不得到了聚丙烯酰胺凝胶电泳显示一条单带的酶，酶液比活为28．09u/mg，提纯了5．32倍，纯酶总收率为36．5％。
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| 关 键 词： | 青霉素酰化酶 渗透压冲击法 分离 纯化 |
| 文章编号： | 1001-8255(2002)04-0161-03 |
| 修稿时间： | 2001-03-05 |
Separation and Purification of Penicillin Acylase from E.coli 108 |
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| WU Jin Xia,ZHANG He Ying. Separation and Purification of Penicillin Acylase from E.coli 108[J]. , 2002, 33(4): 161-163 |
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| Authors: | WU Jin Xia ZHANG He Ying |
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| Abstract: | Penicillin G acylase from E.coli 108 was extracted by osmotic shock method to obtain crude enzyme of high specific activity with total recovery of enzyme activity of 93.3%. The enzyme was purified by means of (NH 4) 2SO 4 fractional precipitation and ion exchange column chromatography on DEAE Cellulose 52 to give a product with a single band on polyacrylamide gel electrophoresis. The speci fic a ctivity, purification and recovery of enzyme activity were 28.09 u/mg, 5.32 fold and 36.5%, respectively. |
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| Keywords: | penicillin acylase osmotic shock method separation purification |
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