地西泮的肠胃循环对中枢抑制作用的影响

研究了小鼠ig不同酸度溶液和活性碳对地西泮肠胃循环的干扰,是否影响其中枢抑制作用。HPLC法测定证实,酸性液体(pH1)ig可使小鼠iv地西泮后1h胃中地西泮排泌量显著增加。以iv地西泮后睡眠持续时间、自主活动抑制及转棒试验跌落率的恢复速度和死亡率为指标,比较了酸性活性碳(2g·kg^-1)、中性活性碳和生理盐水对地西泮中枢抑制作用的影响。结果表明,酸性碳组动物其地西泮所致中枢抑制状态的恢复速度明显加快,死亡率降低。

EFFECT OF ENTEROGASTRIC CIRCULATION OF DIAZEPAM ON ITS CNS INHIBITORY ACTION IN MICE

The present study was designed to examine whether disturbance of the enterogastric circulation of diazepam would obviously affect its inhibitory action on CNS. Intragastric administration of acidic liquid (pH 1) elicited a marked increase in the amount of diazepam in the gastric juice of mice 1 h after i.v. diazepam, as measured by HPLC method. The effects of ig acidic charcoal (2 g.kg-1, pH 1), neutral charcoal and normal saline on diazepam-induced CNS depression were compared by studying the duration of hypnotic action, inhibition of spontaneous activity, dropping rate in rotating rod test and mortality after i.v. diazepam (10-40 mg.kg-1) in mice. The results showed that the animals receiving ig acidic charcoal recovered more rapidly from CNS depression and exhibited lower mortality than the animals in neutral charcoal group and normal saline group.