儿童单纯疱疹病毒感染血清学检查分析

目的：单纯疱疹病毒(HSV)感染是小儿时期常见的病毒感染,也是病毒性脑炎的常见病因,血清学检查是诊断HSV感染常用的检测方法。该文探讨儿童HSV感染的血清抗体检查的意义。方法：采用ELISA方法检测2436例儿童血清中HSV-1和HSV-2的IgG和IgM抗体,并作回顾性分析。结果：儿童单纯疱疹病毒血清抗体阳性率为44.6%(143/321),其中HSV-1抗体阳性率38.9%(117/301),HSV-2抗体阳性率15.9%(43/271);IgM阳性率为41.1%(132/321),IgG阳性率为25.5%(73/286);HSV-1血清阳性率随年龄而增加(P<0.01)。TORCH检测组HSV-2抗体血清阳性率1.9%,均为HSV-2IgG阳性。结论：HSV血清学检查对儿童HSV感染具有重要的诊断意义。

Serologic examination for childhood herpes simplex virus infection

Objective This study was designed to investigate the value of serologic examination in the diagnosis of childhood herpes simplexvirus (HSV) infection.Methods Serum samples were collected from 2 436 outpatients and inpatients. The samples were divided into two groups. Group 1 consisted of 321 samples which were assayed for HSV-1 IgG, HSV-1 IgM, HSV-2 IgG or HSV-2 IgM antibody using herpes simplex virus antibody kits between January 2003 and November 2005. Group 2 consisted of 2 115 samples which were assayed for HSV-2 IgG and IgM antibodies by TORCH testing between October 2004 and November 2005. Results In Group 1, the total seroprevalence of HSV infection was 44.6%, with 38.9% being HSV-1 positive and 15.9% HSV-2 positive; HSV-IgM positivity was found in 41.1% and 25.5% were HSV-IgG positive; HSV-1 seroprevalence significantly increased with age (P <0.05). In Group 2 the seroprevalence of HSV-2 infection was 1.9%; all of the samples were HSV-2 IgG positive.Conclusions HSV serologic examination is useful in the diagnosis of HSV infection in children.