曲安奈德对实验性视网膜静脉阻塞血管内皮生长因子表达的影响

目的:研究曲安奈德(TA)对视网膜静脉阻塞(RVO)大鼠视网膜组织中血管内皮生长因子(VEGF)表达的影响.方法:SD大鼠48只,随机分成3组:空白组(平衡盐液),对照组(模型+平衡盐液),实验组(模型+TA),观察对照组和实验组模型眼底情况.每组分别于玻璃体腔注射平衡盐液或TA后第3、第5、第7、第14天随机处死4只大鼠,分别用免疫荧光组织化学和酶联免疫吸附试验(ELISA)测定VEGF的表达.结果:在RVO的2组模型眼中,实验组眼底病变改善显著,与对照组差异有统计学意义.免疫组织化学图像定量分析和ELISA测定显示在模型眼中VEGF的表达显著增加,与空白组比较差异有统计学意义；实验组VEGF表达在各个时间点上均显著低于对照组.结论:TA明显改善RVO模型眼底病变的作用可能是通过TA下调了视网膜组织中VEGF的表达.

Effect of Triamcinolone Acetonide on Vascular Endothelial Growth Factor Levels in Experimental Retinal Vein Occlusion

Objective：To investigate the molecular mechanism by which triamcinolone acetonide（TA ） may reduce edema in a SD rat model of retinal vein occlusion（RVO）.Methods：After baseline ophthalmoscopic examination and fundus photography, a RVO was created photothrombotically in both eyes of 32 rats,using green photocoagulation and intravenous Rose Bengal. Following this,32 eyes were injected intravitreally with 320μg/8μl TA.After several days,all eyes were re-examined.Fluorescein angiography,in addition to ophthalmoscopy and fundus photography,was performed.Following sacrifice of the animals, the eyes were enucleated and processed,vascular endothelial growth factor（VEGF） was detected by ELISA and fluorescent immunohistochemistry,and compared and analyzed it by light microscope and image analysis software.Results：The changes of eye fundus amelioration were observed obviously in experimental group.Retinal VEGF levels were significantly lower in the TA-treated eyes as compared with the untreated eyes（P〈0.01）.Conclusions：Intravitreal TA can down-regulate VEGF. This event may contribute to a reduction in the blood retinal barrier breakdown that occurs in RVO.