| hBMP7基因转染对兔骨髓间充质干细胞增殖及胶原合成的影响 |
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| 引用本文： | 金丹,裴国献,齐凤菊,胡罢生,魏宽海,王珂,陈滨. hBMP7基因转染对兔骨髓间充质干细胞增殖及胶原合成的影响[J]. 解放军医学杂志, 2002, 27(6): 475-476 |
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| 作者姓名： | 金丹 裴国献 齐凤菊 胡罢生 魏宽海 王珂 陈滨 |
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| 作者单位： | 510515,广州,第一军医大学南方医院全军创伤骨科中心;510515,广州,第一军医大学南方医院全军创伤骨科中心;510515,广州,第一军医大学南方医院全军创伤骨科中心;510515,广州,第一军医大学南方医院全军创伤骨科中心;510515,广州,第一军医大学南方医院全军创伤骨科中心;510515,广州,第一军医大学南方医院全军创伤骨科中心;510515,广州,第一军医大学南方医院全军创伤骨科中心 |
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| 基金项目： | 国家重点基础研究发展规划(编号1999054309)和全军医学科研十五计划重大项目(编号01Z045)资助课题 |
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| 摘 要： | 使用含hBMP7基因的重组逆转录病毒液感染兔骨髓间充质干细胞（BMSc）,MTT法检测细胞增殖能力,流式细胞仪检测细胞周期,^3H脯氨酸掺故法检测I型胶原合成和表达情况。结果显示,经hBMP7基因转染的BMSc与空载体转染及未转染的BMSc在细胞增殖、细胞周期表现方面无显著性差异,经转染的BMSc合成胶原显著高于空载体转染和未转染者。提示采用逆转录病毒介导转染BMSc后能促进细胞的胶原合成,对BMSc增殖和细胞周期无显著影响,有利于进一步 应用于构建组织工程化骨组织。
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| 关 键 词： | 骨形态发生蛋白质类 骨髓间充质干细胞 生物医学工程 |
| 修稿时间： | 2002-05-13 |
HUMAN BONE MORPHOGENETIC PROTEIN 7 EXPRESSION,PROLIFERATION AND COLLAGEN SYNTHESIS OF BONE MARROW STEM CELL TRANSFERRED BY RETROVIRAL VECTOR MEDIATED hBMP7 GENE |
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| Jin Dan,Pei Guoxian,Qi Fengju et al. HUMAN BONE MORPHOGENETIC PROTEIN 7 EXPRESSION,PROLIFERATION AND COLLAGEN SYNTHESIS OF BONE MARROW STEM CELL TRANSFERRED BY RETROVIRAL VECTOR MEDIATED hBMP7 GENE[J]. Medical Journal of Chinese People's Liberation Army, 2002, 27(6): 475-476 |
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| Authors: | Jin Dan Pei Guoxian Qi Fengju et al |
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| Affiliation: | Jin Dan,Pei Guoxian,Qi Fengju et al. Department of Orthopedics and Traumatology,Nanfang Hospital,First Military Medical University,Guangzhou 510515 |
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| Abstract: | The targeted cells: rabbit bone marrow stem cells were infected with hBMP virus granules which secreted from PT67 cells and also selected by G418. The ability of cell proliferation was detected with MTT method and flow cytometer. Synthesis of collagen was assessed by the incoporation of 3 H proline. After infected with virus no significant difference of cell proliferation and cell cycle was found between the cells transferred by PLNCX 2 hBMP 7 ,PLNCX 2 . Significant difference was found in collagen among them. It is suggested that proliferation and cell cycle of the rabbit bone marrow stem cells transferred by the human BMP 7 gene are not affected, and synthesis of bone specificity collagen can be significantly promoted. |
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| Keywords: | bone morphogenetic proteins bone marrow stem cell biomedical engineering |
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