Rapid identification of atypical variant of plasma butyrylcholinesterase by PCR. |
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| Authors: | Franck Ceppa Stephane Gidenne Alain Benois Eleonore Fontan Pascal Burnat |
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| Institution: | Laboratoire de Biochimie, Toxicologie, et Pharmacologie cliniques, H?pital d'Instruction des Armées Bégin, Saint Mandé, France. hiabegin.biochimie@worldonline.fr |
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| Abstract: | Human butyrylcholinesterase is the enzyme responsible of mivacurium and succinylcholine metabolism, which may be significantly impaired when mutation Asp70Gly is found in patients. We describe a simple PCR method for the detection of this variant. Thirteen out of sixteen patients tested after prolonged apnea were positive for the presence of this mutation (50.0% homozygotes and 31.3% heterozygotes), suggesting that this test contributes to the explanation of some clinical events and to their prevention in relatives of these patients. |
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