| 电针对糖尿病大鼠坐骨神经NGF mRNA和IGF-1 mRNA表达的影响 |
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| 引用本文： | 黄召谊,邹欣,王开富.电针对糖尿病大鼠坐骨神经NGF mRNA和IGF-1 mRNA表达的影响[J].中西医结合研究,2010,2(2):57-60. |
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| 作者姓名： | 黄召谊 邹欣 王开富 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院,中西医结合科,武汉,430030
2. 华中科技大学同济医学院附属同济医院,中西医结合研究所,武汉,430030 |
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| 摘 要： | 目的观察电针对糖尿病大鼠坐骨神经组织中神经生长因子(NGF)mRNA和胰岛素样生长因子-1(IGF-1)mRNA表达的影响,初步探讨电针治疗糖尿病周围神经病变的机制。方法SD大鼠115只,随机分成空白组35只、造模组80只。后者经腹腔注射链脲佐菌素(STZ)造模,成模动物共73只。随机抽取70只,分成模型组与电针组各35只,电针组每天电针45min。所有动物分批于试验开始后第1、2、4、6及10周后处死,取坐骨神经,抽提总RNA。采用逆转录-聚合酶链反应技术(RT-PCR)检测坐骨神经组织中NGF、IGF-1的水平。结果模型组NGFmRNA及IGF-1mRNA的表达水平比空白组显著降低(P0.05)。电针组NGFmRNA及IGF-1mRNA的表达随电针的干预时间而逐渐升高;第4周NGFmRNA明显高于空白组和模型组,差异有统计学意义(P0.05),至第10周时仍维持较高水平,明显高于空白组和模型组,差异有统计学意义(P0.05);在第2周时电针组IGF-1mRNA的表达开始显著上升,第4周达到高峰,明显高于模型组,差异有统计学意义(P0.05),至第10周时仍维持较高水平,明显高于空白组和模型组,差异有统计学意义(P0.05)。结论NGFmRNA和IGF-1mRNA在糖尿病大鼠坐骨神经中表达降低,电针刺激促使大鼠坐骨神经的NGFmRNA和IGF-1mRNA的表达增加可能是电针治疗糖尿病神经病变的机制之一。
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| 关 键 词： | 电针 糖尿病大鼠 坐骨神经 神经生长因子 胰岛素样生长因子-1 |
Effect of Electroacupuncture on mRNA Expression of NGF and IGF-1 in Sciatic Nerve of Diabetic Rats |
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| Authors: | HUANG Zhaoyi ZOU Xin WANG Kaifu |
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| Institution: | HUANG Zhaoyi1,ZOU Xin2,WANG Kaifu2 1Department of Integrated Traditional Chinese and Western Medicine,2Institute of IntegratedTraditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective To explore the mechanism of electroacupuncture on diabetic neuropathy,we observed the mRNA expression of NGF and IGF-1 in sciatic nerve of diabetic rats.Methods 115 male SD rats were randomly divided into two groups:blank group(35 rats)and needing model group(80 rats).With streptozotocin(STZ)once intraperitoneal injection in 80 male SD rats,73 rats was duplicated to the diabetic model rats at last.70 diabetic model rats were randomly divided into two groups:electroacupuncture group(35 rats)and mod... |
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| Keywords: | electroacupuncture diabetic rat sciatic nerve nerve growth factor insulin-like growth factor-1 |
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