不同饲料诱发大、小鼠肥胖的比较观察

目的筛选用于建立大鼠和小鼠肥胖模型的高脂饲料配方。方法用5种改进配方的高脂饲料分别喂养大鼠8周，观察其体重、Lee’s指数、脏器系数、腹腔脂肪指数、血脂及血糖等指标，选择高脂饲料C饲喂小鼠8周，观测相应指标。结果与对照组比较，高脂饲料C组大鼠体重、Lee’s指数、肝脏系数、肾脏系数、脾脏系数、睾丸系数、腹腔脂肪指数、甘油三脂、胆固醇、血糖显著增加（P〈0．05）；与对照组比较，高脂饲料C组小鼠体重和心、肝、脾、肾的脏器系数、腹腔脂肪指数、胆固醇及血糖显著增加（P〈0．05），而Lee’s指数、甘油三脂和睾丸脏器系数无显著性变化（P〉0．05）。结论总油脂含量15％和黄豆粉含量24％的高脂饲料配方较为合理，可引起大鼠营齐性肥胖，伴随高脂血症和高血糖；可引起小鼠体重快速增加和脏器系数增加，伴随高胆固醇血症和高血糖。

A Comparative Observation on High Fat Diet-induced Obesity in Rats and Mice

Objective To screen the high-glucose-fat diet for inducing obesity in rats and mice. Methods The rats were fed with five kinds of modified high fat diet for eight weeks, the body weight, Lee＇s index, organ coefficient, abdominal fat index, blood lipid and blood glucose of the rats and mice were determined. The mice were fed with high fat diet C for 8 weeks ,and the corresponding data were determined also. Results Compared with the control group, group C rats fed with high fat diet increase significantly in body weight, Lee＇s index, organ coefficient of the liver, kidney, spleen and testicular, abdominal fat index, triglyceride, cholesterol, blood sugar （P〈0.05）. Compared with the control group, group C mice fed with high fat diet increased significantly in body weight, organ coefficient of heart, liver, spleen, and kidney, abdominal fat index, triglyceride and blood sugar （P〈0.05）, but no significant change （P〉0.05） in Lee＇s index, cholesterol and testicular organ coefficient. Conclusion High-fat diet can cause mice and rats nutritional obesity, and blood sugar was significantly increased. High fat diet formula composed of 15% total fat and 24% soybean meal is more reasonable, and the effects on obesity in rat was more pronounced than in mice.