实验性脑损伤中c-fos的表达与脑水肿的相关性研究

目的探讨脑损伤后c-fos基因和蛋白的表达变化规律与脑水肿之间的关系.方法利用Feeney's自由落体法制作局灶性脑损伤动物模型,应用原位杂交和免疫组化技术检测不同受伤时间段c-fos mRNA和蛋白的表达,同时观察脑组织的水肿情况.结果c-fos mRNA在伤后15 min即有高水平表达,并出现两次表达高峰,分别出现于30 min和16 h.c-fos蛋白随受伤时间的延长表达逐渐增强,在16 h表达最强,随后表达下降,48 h时表达又有升高现象.脑水肿在伤后30 min开始发生,逐渐加重,6 h达高峰,持续至16 h,后逐渐减轻,48 h基本正常.结论脑损伤后c-fos的首次表达高峰可能参与了损伤神经元的修复和生存,与神经细胞的保护机制有关.c-fos第二次表达高峰与脑水肿所致缺血缺氧有关,参与脑损伤后的继发性损害.

The study on correlation between the expression of c-fos and brain edema in experimental brain injuries

Objective To explore the association of c-fos expression and brain edema in the experimental brain injury.Methods Rat focal brain injured models were made using Freeney's method.The expressions of c-fos mRNA and protein were detected by situ hybridization and immunohistochemical method in various periods after brain injury.Results The expressions of c-fos mRNA were identified as early as 15 minutes,and reached maximum levels in 30 minutes and 16 hours after injury.The protein expressions of c-fos were up-regulated according to injury time,and reached maximum in 16 hours,and decreased lately,however,increased in 48 hours.Brain edema occur in 30 minutes after injury,while water content got its peak after 6 hours to 16 hours.Conclusion The first expression maximum of c-fos may have neuroprotective effect on the traumatic rat brain.However,the second expression maximum has correlation to ischemia induced by brain edema,and participates in the secondly brain injury.