纳洛酮对缺血再灌注海马细胞线粒体功能保护作用的研究

目的观察缺血再灌注期间海马细胞内游离钙离子、线粒体膜电位的变化以及纳洛酮对细胞的保护作用。方法20只新西兰大白兔随机分成4组对照组、缺血组、再灌注组、纳洛酮组,每组5只,然后以荧光染色流式细胞仪检测各实验组海马细胞内游离钙离子浓度和线粒体膜电位的变化。结果缺血组、再灌注组海马细胞内游离钙离子浓度显著高于对照组和纳洛酮组;其线粒体膜电位则低于对照组和纳洛酮组;再灌注组游离钙离子浓度明显高于缺血组;其线粒体膜电位则低于缺血组;对照组与纳洛酮组间各指标无显著差异。结论纳洛酮对缺血再灌注引起的海马细胞内钙离子浓度升高有抑制作用,对线粒体膜电位下降有一定的保护作用。

Protective effect of naloxone on mitochondrial function of hippocampal cells injured by ischemia-reperfusion

Objective To study the effect of naloxone on the hippocampal cells of rabbit injured by ischemia and reperfusion.Methods Twenty white rabbits were randomly divided into four groups:control group, ischemia group, reperfusion group and naloxone group. At the end of intervention, the concentration of free calcium ion and mitochondrial membrane potential of hippocampal cells were assayed by flow cytometry.Results The concentrations of free calcium ion of both ischemia group and reperfusion group were significantly higher than those of control and naloxone groups(P<0.01 respectively).Their mitochondrial membrane potentials were lower than those of the latters(P<0.01 respectively).The concentration of free calcium ion of reperfusion group was higher than that of ischemia group and its mitochondrial membrane potential was lower than that of ischemia group(P<0.01).There were no remarkable differences in the concentration of free calcium ion and the mitochondrial membrane potential between the naloxone group and control group.Conclusions Ischemia and reperfusion may lead to rise in concentration of free calcium ion, loss of mitochondrial membrane potential of hippocampal cells. Naloxone may significantly inhibit aforementioned changes. It has protective effect on the mitochondrial function of the hippocampal cells.