Serological and biochemical characterization of the mouse mammary tumor virus with localization of p10.

The identification of a cyclic nucleotide-independent protein kinase in purified adenovirus types 2 and 5 is described. Enzyme activity was detected only in virus preparations which had been mildly disrupted by dialysis against low ionic strength buffers and not in intact virus preparations. Virus polypeptides Ma, V, VI, VII, and X were phosphorylated in vitro. The enzyme transferred the -y-phosphate of ATP to serine and threonine residues of virus proteins. Other proteins (histones, casein, and protamine sulphate) were phosphorylated by the virion protein kinase.