濒危药用植物桃儿七中鬼臼毒素和总木脂素含量测定

目的:测定不同产地桃儿七中鬼臼毒素和总木脂素类成分含量,对其资源利用价值进行评价。方法:采用高效液相色谱法和紫外分光光度法分别测定不同产地共126个桃儿七样品中鬼臼毒素和总木脂素含量,采用参比波长法测定桃儿七中总木脂素类成分的含量。结果:桃儿七中鬼臼毒素和总木质素的含量均以产自甘肃省永登县自然保护区样地的样品含量最高,产自西藏自治区江达同普乡样地的样品含量最低,前者分别为7.40%,20.66%,后者分别为0.40%,5.46%,两者分别相差近19倍和4倍。桃儿七中鬼臼毒素与总木脂素的含量呈显著正相关,且两者的含量表现出海拔较低、纬度较高地区样地的含量较高的特点。结论:2种含量测定方法均简便、快速、准确、重复性好;以鬼臼毒素含量和总木脂素含量为指标评价桃儿七资源利用价值较为客观;本研究的结果,对于桃儿七野生资源采集和栽培生产基地建设的产地选择具有重要的指导意义。

Determination of podophyllotoxin and total lignans in Sinopodophyllum emodi

Objective: To determine the content of podophyllotoxin and total lignans in Sinopodophyllum emodi Ying from different areas and evaluate the resource utilization of this endangered medicinal plant. Method: HPLC and UV spectrophotometry were used to determine the content of podophyllotoxin and total lignans in 126 samples from different habitats and the total lignans content was determined by the reference wavelength method. Result: According to the results,the highest content was determined from the samples from Yongdeng Nature Reserve in Gansu province, and the lowest ones was found in the samples from Tibet. The former's podophyllotoxin and total lignans contents were 7.40% and 20.66%, respectively, which were 19 times and 4 times more than those of the latter. The content of podophyllotoxin and total lignans in S. emodi were significantly positively related, meanwhile, samples from both low altitude and high latitude showed the higher content. Conclusion: The two determination methods are simple, rapid, accurate and repeatable. It is more scientific and rational to evaluate the resource utilization of S. emodi with two indicators, those are the content of podophyllotoxin and the content of total lignans. This paper is instructive to the collection of wild resources and the establishment of production bases.