| 人参皂苷Rg1 PEG修饰及其稳定性实验研究 |
| |
| 引用本文： | 刘梅,王莉,胡凯莉,奉建芳. 人参皂苷Rg1 PEG修饰及其稳定性实验研究[J]. 中国中药杂志, 2012, 37(10): 1378-1382 |
| |
| 作者姓名： | 刘梅 王莉 胡凯莉 奉建芳 |
| |
| 作者单位： | 上海中医药大学穆拉德中药现代化研究中心,上海,201203 |
| |
| 基金项目： | 国家自然科学基金项目(30873445) |
| |
| 摘 要： | 目的:探讨人参皂苷Rg1经PEG修饰前后在大鼠离体胃中的稳定性。方法:取SD大鼠,禁食18 h后,随机分为Rg1组和PEG-Rg1组,准确吸取Rg1胃灌注液和PEG-Rg1胃灌注液注射到离体胃内,37℃恒温振荡,不同时间点取样,采用UPLC对样品中指标成分人参皂苷Rg1进行含量测定,观察比较人参皂苷Rg1与PEG-Rg1在离体胃中的稳定性差异。结果:Rg1在大鼠离体胃中的稳定性差,2 h时测得Rg1为26.8%,降解73.2%,PEG-Rg1在大鼠离体胃中的稳定性提高,2 h时测得Rg1仍有81.8%,仅降解18.2%。结论:PEG修饰之后可以提高人参皂苷Rg1在胃中的稳定性,可以改善修饰之前游离的人参皂苷Rg1在胃中容易降解,稳定性差等问题。
|
| 关 键 词： | 人参皂苷Rg1 PEG修饰 稳定性 |
| 收稿时间： | 2011-12-07 |
Modification and stability of ginsenoside Rg1 PEG |
| |
| LIU Mei,WANG Li,HU Kaili and FENG Jianfang. Modification and stability of ginsenoside Rg1 PEG[J]. China Journal of Chinese Materia Medica, 2012, 37(10): 1378-1382 |
| |
| Authors: | LIU Mei WANG Li HU Kaili FENG Jianfang |
| |
| Affiliation: | Murad Research Center for Traditional Chinese Medicine Modernization, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Center for Traditional Chinese Medicine Modernization, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Center for Traditional Chinese Medicine Modernization, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Center for Traditional Chinese Medicine Modernization, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
| |
| Abstract: | Objective: To study the stability of ginsenoside Rg1 before and after being modified by PEG(PEG-Rg1) in isolated rat stomachs.Method: SD rats,after 18 h of fasting,were randomly divided into the Rg1 group and the PEG-Rg1 group.Rg1 stomach perfusion fluid and PEG-Rg1 infusion fluid were accurately extracted and injected into their stomachs in vitro,with oscillation at 37 ℃.Samples were taken in different time points and contents of ginsenoside Rg1 were determined by UPLC to observe and compare the stability of ginsenoside Rg1 and PEG-Rg1 in rat stomachs in vitro.Result: Rg1 in rat stomachs showed poor stability,Rg1 was measured to be 26.8% in 2 h,with degradation of 73.2%.Its stability in PEG-Rg1 was improved in rat stomachs,Rg1 was measured to be 81.8% in 2 h,with degradation of only 18.2%.Conclusion: PEG-modified ginsenoside Rg1 can enhance the stability of ginsenoside Rg1 in stomach and improve degradation and poor stability of ginsenoside Rg1 in stomach. |
| |
| Keywords: | PEG-modified ginsenoside Rg1 stability |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
