癫(癎)大鼠海马齿状回微管相关蛋白2表达规律及地西泮干预的研究

目的研究致癎大鼠神经元树突标记物微管相关蛋白2(MAP2)的表达规律、与癫癎发生的关系及在地西泮干预下的变化。方法建立戊四氮致癎大鼠模型,分为戊四氮生理盐水组、戊四氮地西泮组、地西泮戊四氮组及正常对照组。采用免疫组化方法观察大鼠癎后海马结构MAP2免疫反应性(MAP2-IR),及其在地西泮干预下的动态变化,用平均吸光度值(COD)表示免疫反应性的强度。结果戊四氮生理盐水组及戊四氮地西泮组海马齿状回分子细胞层MAP2-IR的COD值于癎后3d增强(均P<0.05),7d明显增强(均P<0.01),14d达到高峰(均P<0.01),28d降至正常对照组水平;地西泮戊四氮组未能观察到MAP2的明显变化。结论致癎大鼠海马齿状回MAP2-IR变化显著,癎后的地西泮干预不能改变MAP2-IR的变化规律。

Changing features of microtubule associated protein 2 expression in hippocampal dentate gyrus of epileptic rats and the intervention results after using Diazepam

Objective To investigate the changing features of microtubule associated protein 2 (MAP2) expression, which was a neural dendrite marker, of epileptic rat and the intervention results after using Diazepam. The relations between MAP2 and epileptogenesis were also explored.Methods Model of epileptic rat was established by Pentylenetetrazol (PTZ) and divided into PTZ-NA group,PTZ-Diazepam group, Diazepam-PTZ group and normal control group. Immunohistochemistry method was applied on hippocampus of epileptic rats to determine the change of MAP2 immunoreactivity (MAP2-IR) with or without Diazepam intervention at various time points. MAP2-IR was showed by mean optical density (COD).Results In the PTZ-NA group and PTZ-Diazepam group, MAP2-IR in molecular cell layer of hippocampal dentate gyrus increased after 3 days (all P<0.05), boosted obviously after 7 days (all P<0.01),reached peak after 14 days (all P<0.01) and came back to normal level after 28 days of epilptogenesis. In contrast, no marked change was observed in Diazepam-PTZ team. Conclusions Marked changes of MAP2-IR are found in hippocampal dentate gyrus after PTZ induced seizure, while Diazepam used after seizure has no notable effect on MAP2-IR change.