三氧化二砷联合姜黄素治疗大鼠移植性肝癌的实验研究

目的：观察三氧化二砷联合姜黄素对大鼠移植性肝癌的治疗作用。方法：以直接注射法制备大鼠移植性肝癌模型,120只大鼠随机分为对照组（A组）、三氧化二砷治疗组（B组）、姜黄素治疗组（C组）及三氧化二砷联合姜黄素治疗组（D组）,每组30只,于接种肝癌细胞7天后各组随机处死10只,计算瘤体大小及称肝重量;剩余大鼠经腹腔给药,连续7天。分别测量给药前及停药后14天的体重及血常规,每组选出10只用于记录生存时间,剩余10只大鼠于停药后14天处死,解剖计算瘤体大小及称肝重量。结果：D组瘤体缩小最为显著且与A组、B组及C组有统计学差异（P〈0.05）,A组瘤体变化最小且与B组、C组及D组有统计学差异（P〈0.05）,B组、C组之间差异无统计学意义（P〉0.05）。生存时间B组、C组及D组长于A组（P〈0.05）,D组最长且与B组、C组有统计学差异（P〈0.05）。血常规变化情况在4组间差别无统计学意义。结论：三氧化二砷和姜黄素对大鼠移植性肝癌均有治疗作用,联合用药具有较好的协同效应。

Treatment of Rat Implantation Hepatocarcinoma by Arsenic Trioxide and Curcumin

Objective： To study the treatment effects of arsenic trioxide （As2O3 ） in combination with curcumin （Cur） on implantation hepatocarcinoma of rats. Methods ： The implantation hepatocarcinoma model of rats were made by injecting method. One hundred and twenty rats were divided into group A （ Control group ）, group B （ As2O3 group ）, group C （ Cur group）, group D （combined As2O3 and Cur group） randomly and each group were 30 rats. Ten rats inoculated tumor ceils for 7 days in each group were killed and the tumor volume and liver weight of these rats were measured. The others were administered through abdominal cavity for consecutive 7 days. The weight and blood routine of each rat were measured in preadministration and 14 days of post - administration. The survival lengths of 10 rats in each group were recorded. The tumor volume and liver weight of 10 remnant rats were measured after drug withdrawal 14 days. Results ： The diminution of tumor volume of group D was maximum and different from group A, group B and group C significantly I P 〈 0. 05 ） . Same index of group A was minimum and different from the other three groups significantly （ P 〈 0. 05 ） . Meanwhile, the change of tumor volume of group B was not different from group C （ P 〉 0.05 ） . The survival length of group B, group C and group D was longer than group A （ P 〈 0. 05 ） . Same index of group D was longest and different from goup B and group C remarkably （ P 〈 0. 05 ） . The change of blood routine among four groups was no significant （P 〉 0. 05 ） . Conclusions： Adminstration of As2O3 or Cur has treatment effects on the implantation hepatocarcinoma and combination of the two drugs have better cooperative effect.