硒锌对培养软骨细胞生长代谢的影响

本文报告了硒、锌对培养软骨细胞生长代谢的影响。本实验在培养液中加入一定量硒、锌对一代人胚关节软骨细胞进行培养192h。结果显示:(1)补锌和补硒均能促进软骨细胞的生长。(2)同时补充适量的硒和锌比单独补充效果好,但不能超过正常人血清含量的中等水平,若过多补充则可能适得其反。(3)在同一阶段,软骨细胞的分裂增殖与其产生基质的量不一致。(4)体外培养软骨细胞的基质代谢能很快进入平衡状态。胶原较蛋白多糖的代谢为慢。

Effects of selenium and zinc on the growth metabolism of cultured chondrocytes

In this pater,the effects of selenium andzinc on the growth metabolism of culturedchondrocytes were reported.Human embryoarticular chondrocytes had been recultured inthe media supplied with selenium and zinc for129 hours.The result showed:(1)zinc and se-leneum supplied in the media at a given con-centration could all promot the growthmetabolism of cultured chondrocyte;(2)theeffects were better when both zinc and seleni-um were supplied at the same time.But theconcentration can not be over the mediumlever of normol human serum.The concentra-tion over the normel level lead to the oppositeresult;(3)in the same period,the reproductionof chondrocytes and matrix were not equal inquantity;(4)the matrix metabolism of cul-tured chondrocytes could get balanced quick-ly.The metabolism of the collagen was slowerthan the proteoglycan.