DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine |
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Authors: | E A Mullokandov W A Franklin M Brownlee |
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Institution: | (1) Department of Radiation Oncology and Diabetes Research Center, Albert Einstein College of Medicine, Bronx, NY, USA , US |
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Abstract: | Summary In order to evaluate whether base modifications, apurinic/apyrimidinic site formation, strand breaks, or a combination of
these lesions results from the interaction of glycation products with DNA, plasmid DNA was first reacted with these products,
and then subjected to digestion with endonuclease III and endonuclease IV of Escherichia coli. Analysis of the differential effects of digestions with these enzymes by electrophoresis on agarose gels demonstrated that
reactive glycation products produce both base modification and apurinic/apyrimidinic sites in DNA, in addition to the strand
breaks observed after incubation with glycation products alone. These types of DNA damage may occur in specific diabetic cells
where elevated levels of glycating sugars are associated with pathologic dysfunction. Diabetologia (1994) 37: 145–149]
Received: 28 May 1993 and in revised form: 30 August 1993 |
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Keywords: | Amadori products endonucleases apurinic/apyrimidinic sites base modifications strand breaks |
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