| 乳鼠心肌膜微血管内皮细胞的体外培养 |
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| 引用本文： | 张涛,滕可导,田启超,田丽芳,穆祥. 乳鼠心肌膜微血管内皮细胞的体外培养[J]. 解剖学报, 2008, 39(1): 121-123 |
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| 作者姓名： | 张涛 滕可导 田启超 田丽芳 穆祥 |
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| 作者单位： | 1.中国农业大学动物医学院，北京 100094;2. 四川农业大学动物科技学院，雅安 625014;3.北京农学院动物科学技术系，北京 102206;4.北京农学院兽医学(中医药)北京市重点实验室，北京 102206 |
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| 基金项目： | 北京市自然科学基金 , 北京市兽医学(中医药)重点实验室资助项目 |
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| 摘 要： | 目的 改进心肌膜微血管内皮细胞的体外培养方法,为进一步研究其结构和功能提供实验数据.方法 选取10～15d龄的SD大鼠,采用植块法培养原代心肌膜微血管内皮细胞,在继代培养中通过差速消化和差速贴壁方法、结合倒置显微镜下形态学的观察进行继代培养和纯化,利用免疫细胞化学方法检测第Ⅷ因子相关抗原对培养细胞进行了鉴定.结果 成功培养了大鼠心肌膜微血管内皮细胞,细胞呈多边形或鹅卵石状;免疫细胞化学染色第Ⅷ因子相关抗原显阳性.结论 改良了心肌膜微血管内皮细胞的体外培养方法,获得了较高纯度的心肌膜微血管内皮细胞,可用于进一步的科学研究.
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| 关 键 词： | 心肌膜 微血管内皮细胞 体外培养 乳鼠 |
| 收稿时间： | 2007-03-14 |
| 修稿时间： | 2007-05-10 |
THE CULTURE OF MYOCARDIAL MICROVASCULAR ENDOTHELIAL CELLS OF SUCKLING RATS IN VITRO |
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| ZHANG Tao,TENG Ke-dao,TIAN Qi-chao,TIAN Li-fang,MU Xiang. THE CULTURE OF MYOCARDIAL MICROVASCULAR ENDOTHELIAL CELLS OF SUCKLING RATS IN VITRO[J]. Acta Anatomica Sinica, 2008, 39(1): 121-123 |
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| Authors: | ZHANG Tao TENG Ke-dao TIAN Qi-chao TIAN Li-fang MU Xiang |
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| Affiliation: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China; 2. College of Animal Science and Technology, Sichuan Agricultural College, Yaan 625014, China; 3. Department of Animal Science and Technology;4. Beijing Key Laboratory of Veterinary Medicine Graditional Chinese Medicine and Pharmacology, Beijing Agricultural College, Beijing 102206, China |
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| Abstract: | Objective To improve methods of culturing rat myocardial microvascular endothelial cells (RMMVECs) EM>in vitro/EM> and provide experimental data for studying the structures and functions of RMMVECs. Methods SD rats of 10_15 days old were selested. Explants cultivation of cardiac tissues was used to obtain the primary cells. In the subculturing, the cell cultures were purified by different speeds of attachment and digestion from contaminant cells combined with morphological observation. The purified cells were identified as endothelial cells by morphology and by positive immunocytochemistry for factor Ⅷ related antigen, a marker for endothelial cells. Results The RMMVECs were cultured and purified successfully which grew in a polygonal or cobblestone pattern and were positive for the immunocytochemical staining of factor Ⅷ related antigen. Conclusion The methods of culturing RMMVECs were improved, RMMVECs with high purity were o |
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| Keywords: | Myocardium Microvascular endothelial cells In vitro culture Suckling rat |
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