急进高原致大鼠空间记忆能力障碍的研究

目的：探讨急进高原对于大鼠空间记忆能力的影响。方法：48只SD大鼠随机分为3组：平原组（对照组）、急进海拔4000m组（4km组）、急进海拔6000m组（6km组）。根据大鼠Morris水迷宫定位导航训练在急进高原饲养之前和之后,将实验分为两部分（每部分实验使用不同的大鼠）,统计分析各实验组大鼠逃避潜伏期、游泳总路程、60s内穿越平台次数和目标象限滞留时间,并分别与对照组进行比较。结果：当大鼠Morris水迷宫定位导航训练在急进高原饲养之前时,急进高原饲养后,6km组大鼠较对照组60s内穿越平台次数显著减少（P〈0.05）,目标象限滞留时间显著缩短（P〈0.01）。当大鼠Morris水迷宫定位导航训练在急进高原饲养之后时,6km组大鼠较对照组第7天平均逃避潜伏期和平均游泳总路程显著缩短（P〈0.01、P〈0.05）;空间探索测试中,6km组大鼠较对照组60s内穿越平台次数显著减少（P〈0.05）,目标象限滞留时间显著缩短（P〈0.01）。结论：急进海拔6000m以上将导致大鼠空间记忆能力障碍。

Spatial memory impairment induced by rapid ascent to high altitude in rats

Objective：To investigate the effects of rapid ascent to high altitude on spatial memory impair- ment in rats. Methods： A total of 48 Sprague--Dawley rats were randomly divided into three groups, the champaign group （control group）, rapid ascent to 4000 meters group （4km group） and rapid ascent to 6000 meters group （6 km group）. According to Morris water maze navigation test for rats before and after the rapid ascent to high altitude, the experiment was divided into two parts （each part of the experiment using different rats）. For each group, the escape latency （s） and path--length （cm） to find the platform, num- ber of times of crossing the former platform location and swimming time in the target quadrant within 60 s were statistically analyzed and compared with the control group respectively. Results： When the Morris water maze navigation test for rats on the rapid altitude, number of times of crossing the former ascent to high altitude before, after rapid ascent to high platform location within 60 s was significantly reduced （P 〈0. 05） and swimming time in the target quadrant within 60 s was significantly shorter （P〈0.01） in the 6kin group as compared to those in the control group. When the Morris water maze navigation test for rats after rapid ascent to high altitude, the average escape latency and path--length to find the platform on the 7th day were significantly shorter （P〈0. 01, P〈0. 05） and in the space exploration experiment, number of times of crossing the former platform location ming time in the target quadrant within 60 s was pared to those in the control group. Conclusion： within 60 s was significantly reduced （P〈0.05）, swim- significantly shorter （P〈0.01） in the 6km group as corn- Rapid ascent to above 6000 meters will result in spatial memory impairment in rats.