HLA-DPB1等位基因与四川汉族人哮喘的相关性研究

目的:探讨HLA-DPB1等位基因与哮喘之间是否存在相关性,从基因水平探讨支气管哮喘的发病机制。方法:用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)分析法,检测四川汉族人HLA-DPB1基因多态性。用PCR技术对HLA-DPB1基因第2外显子(exon 2)进行体外扩增;然后用Ban II、Fok I、Dde I、Rsa I、EcoN I、BstU I 6种限制性内切酶对扩增产物进行酶切,电泳分离酶切片段,根据片段格局确定相应基因型别。结果:PCR扩增在327 bp有HLA-DPB1 exon 2目的基因产生。限制性内切酶酶切HLA-DPB1后,共检测到12种等位基因。HLA-DPB1*0201在哮喘患者组的出现频率显著低于对照组(P<0．05,RR=0．115<1)。结论:HLA-DPB1* 0201可能为支气管哮喘的抗性基因。

A Study on the Associations between HLA-DPB1 Loci and Asthma of Sichuan Hans'''' Individuals

Objective:In order to study certain HLA-DPBl alleles association with asthma and mechanism of asthma in gene level. Methods: HLA-DPB1 exon 2 gene was cloned in PCR. PCR products were electrophoresed in 2% agarose gel to validate the motive gene. Then HLA-DPB1 exon 2 gene was each cleaved by six restriction endonucleases including Ban II, Fok I, Dde I, Rsa I, EcoN I, BstU I and was directly electrophoresed according to RFLP. The HLA-DPBl alleles were acquired, and the frequencies of them were compared between patients and normal controls. Results: The second DPB1 exon 2 and HGH gene were successfully amplified by PCR. The amplified DNAs were digested with restriction endonucleases. Here we got twelve DPB1 alleles . There was significantly decreased gene frequency of HLA-DPBl * 0201 in asthmatic patients compared with normal controls (P<0. 05, RR = 0. 115<1). Conclusion: HLA-DPBl * 0201 could be acting as IS gene and confer resistense to asthma.