肿瘤坏死因子-α、白介素-6在老年代谢综合征中的检测以及与胰岛素抵抗的相关性研究

目的：探讨代谢综合征（MS）患者在不同时期血清肿瘤坏死囚子-α（TNF-α）、白介素-6（IL-6）浓度变化的意义以及TNF-α、IL-6与胰岛素抵抗（IR）的相互关系。方法：90例老年肥胖患者根据糖脂代谢紊乱程度分为代谢综合征合并2型糖尿病（T2DM）组、肥胖合并T2DM组、单纯肥胖组各30例。30例健康者为健康对照组。所有受试者均采用酶联免疫吸附法检测血清TNF-α、IL～6水平。结果：①肥胖各组TNF-α、IL-6均较健康对照组明显升高（P〈0．01），且TNF-α、IL-6在单纯肥胖组、肥胖合并T2DM组、MS合并T2DM组呈逐渐升高，组间比较差异有统计学意义（P〈0．01）。②血清TNF-α、IL-6与稳态模型胰岛素抵抗指数（HOMA—IR）呈正相关关系（P〈0．01）。结论：代谢综合征患者血清TNF-α、IL-6浓度与MS的程度相关，可引起胰岛素抵抗的发生。

Relationship between the Biologic Assay for Tumor Necrosis Factor Alpha and Interleukin-6 in Elderly Patients with Metabolic Syndrome and Insulin Resistance

OBJECTIVE ： To explore the changes of levels of tumor necrosis factor - α（ TNF - α） and interleukin - 6 （ IL - 6） at different stages of metabolic syndrome （MS） in the elderly patients and the relationship between TNF - α and IL - 6 and insulin resistance （IR）. METHODS： Based on the degrees of disorders of glycolipid metabolism, 90 elderly patients with obesity were divided into three groups including MS combined with type 2 diabetes mellitus （ T2DM ） group （ 30 cases ）, obesity combined with T2DM group （30 cases） and healthy elderly subjects （control group）. Serum concentrations of TNF -α and IL -6 were measured by an enzyme - linked immunosorbent assay （ELISA）. RESULTS ： （ 1 ） Serum concentrations of TNF - α and IL - 6 were obviously increased among obesity groups compared with control group. Serum levels of TNF - α and IL - 6 were gradually increased among all the groups. There were significant differences among all the groups （P 〈 0. 01 ）. （2） Serum concentrations of TNF-α and IL- 6 were correlated positively with the level of HOMA - IR. CONCLUSION ： Serum concentrations of TNF - α and IL - 6 in elderly patients with MS are associated with the presence of MS, which induces IR in the elderly.