Periodontal tissue engineering after tooth replantation |
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Authors: | Reichert da Silva Assunção Luciana Colenci Renato Ferreira do-Amaral Caril Constante Sonoda Celso Koogi Mogami Bomfim Suely Regina Okamoto Roberta de Assis Golim Marjorie Deffune Elenice Percinoto Célio Penha de Oliveira Sandra Helena |
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Institution: | School of Dentistry, Federal University of Pará, UFPA, Pará, Brazil. lurassuncao@yahoo.com.br |
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Abstract: | Background: Blood‐derived products, platelet‐poor plasma (PPP) and platelet‐rich plasma (PRP), constitute an approach in the enhancement of tissue healing. PRP has also been used as a scaffold for bone marrow stem cells in tissue engineering. This study evaluates the effect of PPP, calcium chloride–activated PRP (PRP/Ca), calcium chloride– and thrombin‐activated PRP (PRP/Thr/Ca), and bone marrow mononuclear cells and PRP/Ca (BMMCs/PRP/Ca) on the healing of replanted dog teeth. Methods: After 30 minutes of extraction, teeth were replanted with 1) no material (control); 2) PPP; 3) PRP/Ca; 4) PRP/Thr/Ca; or 5) BMMCs/PRP/Ca. Histologic, histomorphometric, and immunohistochemical analysis was assessed 120 days after replantation. Data from histomorphometric analysis were analyzed statistically (analysis of variance, Tukey; P <0.05). Quantitative immunohistochemical analysis was analyzed by Kruskal‐Wallis and Dunn post hoc test (P <0.05). Results: Flow cytometry analysis showed 55.98% of CD34+ and 32.67% of CD90/Thy‐1 for BMMCs sample. BMMCs/PRP/Ca presented the largest areas of replacement resorption characterized by osseous ingrowth into cementum (P <0.05), with intense immunomarcation for tartrate‐resistant acid phosphatase. The PRP/Ca group also showed areas of replacement resorption with significant immunomarcation for osteopontin. PRP/Thr/Ca presented no replacement resorption. PPP showed areas of inflammatory resorption, with immunomarcation for tartrate‐resistant acid phosphatase. Conclusions: The results suggest that platelets activated with thrombin play an important role in the healing of tissues after tooth replantation. Additional studies are necessary to test other materials, because PRP/Ca did not present an appropriate scaffold for undifferentiated cells in the treatment of avulsed teeth. |
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Keywords: | Bone marrow platelet‐rich plasma stem cells tissue engineering tooth replantation |
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