Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using a Synthetic Convergent Peptide Library, or Mixotope, for Diagnosis of Primary EBV Infection |
| |
| Authors: | D. Tranchand-Bunel H. Gras-Masse B. Bourez L. Dedecker C. Auriault |
| |
| Affiliation: | Laboratoire d'Immunologie Cellulaire, CNRS UMR 8527, Institut Pasteur de Lille, 59019 Lille, France. d.bunel@infobiogen.fr |
| |
| Abstract: | An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive. |
| |
| Keywords: | |
|
|
