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| 日本血吸虫重组蛋白SjGST的表达纯化及单克隆抗体的制备和特性鉴定 | |
| 引用本文： | 刘文,游牧,饶圣宏,徐振山,宋礼华. 日本血吸虫重组蛋白SjGST的表达纯化及单克隆抗体的制备和特性鉴定[J]. 蚌埠医学院学报, 2010, 35(12): 1197-1199. DOI: 10.3969/j.issn.1000-2200.2010.12.001 |
| 作者姓名： | 刘文 游牧 饶圣宏 徐振山 宋礼华 |
| 作者单位： | 安徽医科大学,生物化学与分子生物学教研室,安徽,合肥,230032;安徽大学,生命科学院,安徽,合肥,230039;安科生物工程(集团)股份有限公司,安徽,合肥,230088;安徽医科大学,生物化学与分子生物学教研室,安徽,合肥,230032;安科生物工程(集团)股份有限公司,安徽,合肥,230088 |
| 基金项目： | 国家高技术研究发展计划(863计划)资助项目 |
| 摘 要： | 目的：获取高纯度的日本血吸虫重组GST蛋白（rSjGST）,并制备单克隆抗体。方法：纯化rSjGST后,以其作为抗原,免疫Balb/c雌鼠,并用杂交瘤技术制备抗rSjGST的单克隆抗体,以ELISA方法测定抗体的效价,以蛋白质免疫印迹法测定抗体的特异性。结果：纯化出大量的高纯度rSjGST,并且筛选出能够稳定分泌抗rSjGST单抗的杂交瘤细胞株3C12。用试剂盒检测出单抗的亚型为IgG1。结论：依靠大肠埃希菌表达系统,高效表达出rSjGST,成功制备单克隆抗体,为血吸虫病免疫诊断提供了研究基础。 |
| 关 键 词： | 日本血吸虫 SjGST蛋白 单克隆抗体 |
Purification of recombinant Schistosoma japonicum protein SjGST and preparation and characterization of the monoclonal antibody |
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| LIU Wen,YOU Mu,RAO Sheng-hong,XU Zhen-shan,SONG Li-hua. Purification of recombinant Schistosoma japonicum protein SjGST and preparation and characterization of the monoclonal antibody[J]. Journal of Bengbu Medical College, 2010, 35(12): 1197-1199. DOI: 10.3969/j.issn.1000-2200.2010.12.001 | |
| Authors: | LIU Wen YOU Mu RAO Sheng-hong XU Zhen-shan SONG Li-hua |
| Affiliation: | LIU Wen1,YOU Mu1,RAO Sheng-hong2,XU Zhen-shan3,SONG Li-hua1,3(1.Department of Biochemistry and Molecule Biology,Anhui Medical University,Hefei Anhui 230032,2.College of Life Science,Anhui University,Hefei Anhui 230039,3.Anhui Anke Biotechnology(Group) Co,Ltd,Hefei Anhui 230088,China) |
| Abstract: | Objective:To isolate and purify recombinant Schistosoma japonicum(Chinese strain) protein glutathione S-transferase(rSjGST) for preparing the monoclonal antibodies(McAbs) against rSjGST.Methods:Balb/c mice were immunized with purified rSjGST protein.The anti-rSjGST monoclonal antibody was obtained through hybridoma technique.Characterization of the antibody was performed by ELISA and Western blot analysis.Results:The data had shown that high purified rSjGST protein and the hybridoma cell line 3C12 secreting... |
| Keywords: | Schistosoma japonicum protein glutathione S-transferase monoclonal antibody |
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