四氯化碳诱导的肝硬化小鼠肝部分切除后肝再生模型的建立及评价

目的：建立并评价肝硬化小鼠的肝部分切除后肝再生模型。方法：50只小鼠随机分成实验组和对照组，每组25只。实验组小鼠腹腔注射四氯化碳（CCl₄），每周2次，8周后形成肝硬化模型，对照组小鼠腹腔注射豆油。2组小鼠均行37%肝部分切除术。观察2组小鼠的手术成功率、术后存活率、术后生存情况和术后肝再生率；HE染色和Masson染色观察2组小鼠肝组织的病理学表现；ELASA法观察2组小鼠血清肝细胞生长因子（HCG）和丙氨酸氨基转移酶（ALT）水平。结果：2组小鼠手术成功率均为100%，术后6周存活率均为100%。术后3周内实验组小鼠肝再生率低于对照组，术后3周时两者基本一致，术后3周之后实验组小鼠的肝再生率高于对照组。HE染色和Masson染色，CCl₄注射8周后肝脏呈肝硬化病理改变，肝部分切除术后肝组织结构逐渐改善。实验组小鼠血清HGF水平明显高于对照组，术后2组小鼠血清HGF水平均较术前有所升高，随后恢复至术前水平。与对照组比较，肝切除后实验组小鼠血清ALT水平升高明显，持续时间较长且到达峰值时间延迟。结论：CCl₄诱导肝硬化小鼠采用丝线结扎后切除左外叶的方法极易建立稳定、可靠的肝硬化小鼠肝部分切除后肝再生模型。

Establishment of liver regeneration model of cirrhosis mice induced by CCl4 after partial hepatectomy and its evaluation

Objective:To establish and evaluate the model of liver regeneration of the cirrhosis mice after partial hepatectomy.Methods:Fifty mice were randomly divided into experiment group (n=25)and control group (n=25).The mice in experiment group were injected with CCl4 intraperitoneally twice per week for 8 weeks and the mice in control group were inj ected with soya oil. Partial hepatectomy of 3 7% liver was performed on all mice in two groups.The successful rate of operation,survival rate after operation,survival statue after operation and postoperative liver regeneration rate of the mice in two groups were observed;the pathologic characteristics of liver tissue of the mice in two groups were observed by HE staining and Masson staining;the levels of serum hepatocyte growth factor (HCG)and alanine aminotransferase (ALT)of the mice in two groups were detected by ELASA methods.Results:The successful rates of operation of the mice in two groups were both 100%,and the survival rates of the mice at 6 weeks after operation were 100%.During 3 weeks after operation,the regeneration rates of liver tissue of the mice in experiment group were lower than those in control group;the regeneration rate of liver tissue of the mice in experiment group was equal to that in control group at 3 weeks after operation;the regeneration rates of liver tissue of the mice in experiment group were higher than those in control group from 3 weeks after operation.The HE staining and Masson staining results showed that the liver tissue presented the pathological changes of cirrhosis 8 weeks after inj ection with CCl4;after partial hepatectomy,the structures of liver tissue were gradually improved.The levels of serum HGF of the mice in experiment group were significantly higher than those in control group;the levels of serum HGF of the mice in two groups were increased compared with the preoperative levels,and then recovered to the preoperative level.Compared with control group,the levels of serum ALT of the mice in experiment group after hepatectomy were increased significantly,and the duration was longer and the time to reach the peak was delayed.Conclusion:It is extremely easy to establish a stable and reliable liver regeneration model of liver cirrhosis mice after partial hepatectomy by the resection of left lateral lobe of the cirrhosis mice induced by CCl4 with silk ligation.