| 外源性IL-2对小鼠CD4^＋CD25^＋调节性T细胞影响的实验研究 |
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| 引用本文： | 姜晓峰,郭大伟,朱磊,崔哲铭,孙文郁,林琳,王学范,唐裕福,梁健. 外源性IL-2对小鼠CD4^＋CD25^＋调节性T细胞影响的实验研究[J]. 中国现代普通外科进展, 2011, 14(8): 594-597. DOI: 10.3969/j.issn.1009-9905.2011.08.002 |
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| 作者姓名： | 姜晓峰 郭大伟 朱磊 崔哲铭 孙文郁 林琳 王学范 唐裕福 梁健 |
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| 作者单位： | 中国医科大学附属第四医院普外科,辽宁沈阳,110032 |
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| 基金项目： | 辽宁省教育厅高等学校科研资助项目(2008824) |
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| 摘 要： | 目的：探讨IL-2对CD4＋CD25＋调节性T细胞（Tregs）的增殖及功能的影响。方法：提取B6小鼠脾脏细胞,流式细胞仪分离CD4＋CD25＋Tregs,将新鲜分离的CD4＋CD25＋Tregs与抗CD3单克隆抗体、同种同系抗原递呈细胞（APCs）及外源性IL-2共同培养,测定其增殖活性;并检测体外扩增后的CD4＋CD25＋Tregs的免疫抑制活性及其Foxp3的表达。结果：与外源性IL-2共同培养的CD4＋CD25＋Tregs增殖程度强烈,与对照组比较,差异有统计学意义（P〈0.05）;体外扩增的CD4＋CD25＋Tregs抑制CD4＋CD25-T细胞增殖活性的能力与新鲜分离的CD4＋CD25＋Tregs相似（P〉0.05）。体外扩增的CD4＋CD25＋Tregs的Foxp3表达与新鲜分离的CD4＋CD25＋Tregs亦相似（P〉0.05）。结论：外源性IL-2能够消除CD4＋CD25＋Tregs的无反应状态,且体外扩增的CD4＋CD25＋Tregs保持了其抑制活性。
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| 关 键 词： | CD4＋CD25＋调节性T细胞 白介素2 免疫抑制 |
Impact of exogenous IL-2 the CD4~+CD25~+ regulatory T cells of mice |
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| JIANG Xiao-feng,GUO Da-wei,ZHU Lei,CUI Zhe-ming,SUN Wen-yu,LIN Lin,WANG Xue-fan,TANG Yu-fu,LIANG Jian. Impact of exogenous IL-2 the CD4~+CD25~+ regulatory T cells of mice[J]. Chinese Journal of Current Advances in General Surgery, 2011, 14(8): 594-597. DOI: 10.3969/j.issn.1009-9905.2011.08.002 |
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| Authors: | JIANG Xiao-feng GUO Da-wei ZHU Lei CUI Zhe-ming SUN Wen-yu LIN Lin WANG Xue-fan TANG Yu-fu LIANG Jian |
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| Affiliation: | JIANG Xiao-feng,GUO Da-wei,ZHU Lei,CUI Zhe-ming,SUN Wen-yu,LIN Lin,WANG Xue-fan,TANG Yu-fu,LIANG Jian Department of Genaral Surgery,the Fourth Affiliated Hospital,China Medical University(Shenyang 110032,China) |
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| Abstract: | Objective: To examine the effection of IL-2 on the proliferation and function of CD4+CD25+ regulatory T cells.Methods: The CD4+CD25+ T cells were isolated from B6 mice spleens using FACS.The freshly isolated CD4+CD25+ T cells were cultured with anti-CD3 monoclonal antibody(mAb),syngeneic antigen presenting cells(APCs) and IL-2.Poliferative responses were measured by thymidine incorporation.The suppressive capability and the Foxp3 expression of in vitro-expanded CD4+CD25+ T cells was also investigated.Result... |
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| Keywords: | CD4＋CD25＋ regulatory T cells·IL-2·Immunosuppression |
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