基泰体外对慢性乙型肝炎患者树突状细胞功能影响的初步研究

目的:探讨基泰对慢性乙型肝炎(简称慢乙肝)患者(chronic hepatitis B,CHB)树突状细胞(dentritic cell,DC)生物学性状的影响.方法:分离慢乙肝患者外周血单核细胞,在含GM-CSF/IL-4及不同浓度的基泰培养条件下制备DC.观察DC形态学变化,流式细胞仪(FCM)测其细胞表型分子CD1a,CD83,CD80及HLA-DR的表达,MTT法测定DC刺激同种异体淋巴细胞增殖能力.结果:与对照组相比,实验组倒置显微镜观察显示出典型的DC形态.基泰(100 μg/mL)处理组的DC膜表面分子CD1a及CD80表达均增高(P<0.05);刺激同种异体淋巴细胞增殖能力(SI)增强(P<0.05).结论:基泰一定程度上可改善CHB的DC免疫学活性.

Effects of Jitai on function of dendritic cells derived from patients with chronic hepatitis B

Objective：To investigate the effects of Jitai on the function of dendritic cells derived from patients with chronic hepatitis B（CHB）.Methods：Dendritic cell（DC） derived from monocyte of the patients with chronic HBV infection and cultured with IL-4 /GMCSF were treated with different Jitai concentrations on 4 d.Cell morphology was monitored under light microscopy.Cell surface molecules including HLA-DR,CD80,CD83,CD1a were assayed by flow cytometry.T cell proliferation induced by DC was assayed by methyl thiazolyl tetrazolium（MTT）.Results：The stimulatory capacity of Jitai（100 ug/mL） in the allogeneic mixed leukocyte reaction（All MLR） markedly enhanced compared with the control group（P0.05）.The expressions of CD1a on DC treated with Jitai was significantly higher than those of DC untreated with Jitai（P0.05）,so was the expression of CD80（P0.05）.Conclusion：Jitai in certain concentration can improve the immunology function of DC derived from CHB.