FGF21 protects human umbilical vein endothelial cells against high glucose-induced apoptosis via PI3K/Akt/Fox3a signaling pathway |
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Authors: | Dongmin Guo Lele Xiao Huijun Hu Mihua Liu Lu Yang Xiaolong Lin |
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Institution: | 1. Department of Pathology, Huizhou Third People''s Hospital, Guangzhou Medical University, Huizhou City, Guangdong Province 516001, China;2. Key Laboratory for Arteriosclerology of Hunan Province, Institute of Cardiovascular Disease, University of South China, Hengyang City, Hunan Province 421001, China;3. Huzhou University, Huzhou City, Zhejiang Province 313000, China;4. Centre for Lipid Research & Key Laboratory of Molecular Biology for infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of infectious Disease, The Second Affiliated Hospital, Chongqing Medical University, Chongqing City 400016, China |
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Abstract: | AimsDiabetic macroangiopathy is the main cause of morbidity and mortality in patients with diabetes. Endothelial cell injury is a pathological precondition for diabetic macroangiopathy. Fibroblast growth factor 21 (FGF21) is a key metabolic regulator which has recently been suggested to protect cardiac myocytes and vascular cells against oxidative stress-induced injury in vitro and vivo. In this study, we aimed to investigate the protective capacity of FGF21 in human umbilical vein endothelial cells (HUVECs) against high glucose (HG)-induced apoptosis via phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt)/FoxO3a pathway.MethodsThe cell viability was examined by CCK-8 assay, Intracellular ROS levels were measured by the detection of the fluorescent product formed by the oxidation of DCFH-DA, Apoptosis was analyzed using Hoechst 33258 nuclear staining and Flow Cytometry Analysis (FCA), the expression of protein were detected by Western blot.ResultsResults show that pretreating HUVECs with FGF21 before exposure to HG increases cell viability, while decreasing apoptosis and the generation of reactive oxygen species. Western blot analysis shows that HG reduces the phosphorylation of Akt and FoxO3a, and induces nuclear localization of FoxO3a. The effects were significantly reversed by FGF21 pre-treatment. Furthermore, the protective effects of FGF21 were prevented by PI3K/Akt inhibitor LY294002.ConclusionsOur data demonstrates that FGF21 protects HUVECs from HG-induced oxidative stress and apoptosis via the activation of PI3K/Akt/FoxO3a signaling pathway. |
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Keywords: | FGF21 eNOS Oxidative stress Apoptosis FoxO3a Akt |
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