| 南北苍术的RAPD分析及其划分的初步探讨 |
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| 引用本文： | 郭兰萍,黄璐琦,王敏,冯学峰,付桂芳,阎玉凝. 南北苍术的RAPD分析及其划分的初步探讨[J]. 中国中药杂志, 2001, 26(3): 156-158 |
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| 作者姓名： | 郭兰萍 黄璐琦 王敏 冯学峰 付桂芳 阎玉凝 |
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| 作者单位： | 中国中医研究院 中药研究所，北京 100700 |
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| 基金项目： | 国家自然科学基金重点资助项目(39730500) |
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| 摘 要： | 目的 :用RAPD方法在分子水平上对南北苍术的划分进行初步探讨。方法 :用 40个引物对 7个居群的南苍术、北苍术及关苍术进行RAPD分析。RAPD分析软件RAPDistance Package———Version 1.04中Dice的方法计算任意两个样品间的遗传距离 ,得树系图。结果 :共检测到多态位点 60个。聚类分析显示 ,苍术有以地域为界聚类的倾向 ,且南苍术首先与关苍术聚类后再与北苍术聚类。结论 :苍术的化学成分、遗传分化和地理分布有一定的相关性 ,以地域为界划分南北苍术有一定道理。
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| 关 键 词： | 道地药材 南苍术 北苍术 关苍术 RAPD |
| 文章编号： | 1001-5302(2001)03-0156-03 |
| 收稿时间： | 2000-07-03 |
| 修稿时间： | 2000-07-03 |
A Preliminary Study on Relationship Between Atractylodes lancea and A. chinensis as Analyzed by RAPD |
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| GUO Lan-ping;HUANG Lu-qi;WANG Min;FENG Xue-feng;FU Gui-fang;YAN Yu-ning. A Preliminary Study on Relationship Between Atractylodes lancea and A. chinensis as Analyzed by RAPD[J]. China Journal of Chinese Materia Medica, 2001, 26(3): 156-158 |
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| Authors: | GUO Lan-ping HUANG Lu-qi WANG Min FENG Xue-feng FU Gui-fang YAN Yu-ning |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700. |
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| Abstract: | Objective: To study the relationship between A. lancea and A. chinensis by RAPD analysis.Method: Using 10 random primers, RAPD of seven Atractylodes samples was performed. The result was analyzed by RAPD is tance Package Version 1.04.Result: A total of 60 polymerphic loci were amplified.It appears that the Atractylodes samples tend to cluster in relation to their habitats,and A.lancea has closephylogenetic relationship with A. japonica.Conclusion: Acertain correlation among the chemical composition, genetic diversity and geographic distribution has been revealed.It is reasonable to separate A.lancea from A.chinensis according to their habitats. |
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| Keywords: | authentic medicinal herbs Atractylodes lancea A. chinensis A. japonica RAPD |
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