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| 活化星形胶质细胞去抑制调控小鼠视神经再生的实验研究 | |
| 引用本文： | 范志刚,Dongfeng Chen,葛坚. 活化星形胶质细胞去抑制调控小鼠视神经再生的实验研究[J]. 眼科学报, 2005, 21(4): 170-172 |
| 作者姓名： | 范志刚 Dongfeng Chen 葛坚 |
| 作者单位： | 1. 眼科学教育部重点实验室,广州,510060;中山大学中山眼科中心,广州,510060 2. 中山大学中山眼科中心,广州,510060 3. 眼科学教育部重点实验室,广州,510060 |
| 摘 要： | 目的:揭示活化星形胶质细胞去抑制对小鼠视神经再生的作用。方法:将Bcl-2高表达转基因小鼠(Bcl-2tg)和GFAP/Vimentin双基因敲除小鼠(GFAP-/-/Vim-/-)交配获得三基因突变小鼠(Bcl-2tg/GFAP-/-/Vim-/-,3M)。分别选取出生后5d(P5)小鼠3只及出生后18d(P18)小鼠3只制作标准的完全性视神经嵌断模型。应用GAP43免疫荧光染色特异性检测再生轴突。结果:Bcl-2tg/GFAP-/-/Vim-/-小鼠在P5时获得视神经再生,再生轴突可以延伸达视交叉水平,而P20时仅获得局部出芽再生。结论:在Bcl-2转基因使视网膜节细胞重获内源性再生能力基础上,通过活化星形胶质细胞去抑制可以促进视神经轴突再生,提示活化星形胶质细胞是抑制视神经再生的重要因素,从而为青光眼等视神经病变的再生治疗提出了新的方向。 |
| 关 键 词： | 青光眼 视神经再生 星形胶质细胞 转基因小鼠 基因敲除小鼠 |
Experimental Modulation of Optic Nerve Regeneration by Reducing Inhibitory Effects of Reactive Astrocytes |
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| Zhigang Fan,Dongfeng Chen,Jian Ge. Experimental Modulation of Optic Nerve Regeneration by Reducing Inhibitory Effects of Reactive Astrocytes[J]. Eye science, 2005, 21(4): 170-172 | |
| Authors: | Zhigang Fan Dongfeng Chen Jian Ge |
| Affiliation: | 1. Key Laboratroy of Ophthalmology of the Ministry of Education and Zhongshan Ophthalmic Center, Sun Yatsen University, Guangzhou 510060, China; 2. Schepens Eye Research Institute, Program in Neuroscience, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA |
| Abstract: | Purpose: To modulate optic nerve regeneration by reducing the inhibitory effects of reactive astrocytes. Methods: Bcl-2 over-expression transgenic mice were mated with GFAP/Vimentin double gene knock out mice (GFAP-/-/Vim-/-) to produce Bcl-2 tg/GFAP-/-/Vim-/- triple mutant mice (3M). Optic nerve crush model was established respectively in 3 P5 and 3 P18 mice. GAP43 immunofluore-scence was used to specifically label regenerative axons. Results: Optic nerve regeneration was observed in P5 3M mice all the way to optic chiasm in P18 3M mice, only local sprouting regeneration was observed. Conclusions: With restablishment of intrinsic ability of retinal ganglian cells by Bcl-2 transgenic, optic nerve regeneration is promoted by reducing the inhibitory effects of reactive astrocytes, which demonstrates reactive astrocyte as an important factor inhibiting optic regeneration and provides new direction for regenerative therapy in glaucoma. Eye Science 2005;21:170-172. |
| Keywords: | glaucoma optic nerve regeneration astrocyte transgenic mice gene knockout mice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |