P27基因在原发性肝癌中的表达及其与预后的关系

目的　探讨 P2 7在原发性肝癌中表达 ,揭示其与原发性肝癌临床病理指标和预后的关系。方法　采用免疫组化二步法 ,检测 4 3例原发性肝癌标本、2 1例肝硬化标本和 16例正常肝脏组织中的 P2 7表达情况 ,并对2 9例肝癌患者进行了随访。结果　 P2 7在正常肝脏组织中少有表达 ,肝硬化组阳性表达有所增加 ,但两组之间差异无显著性 (P>0 .0 5 ) ;P2 7在原发性肝癌组中阳性表达率为 86 .0 4 % ,与肝硬化组和正常对照组相比差异有显著性(P<0 .0 1)。肿瘤直径 >5 cm、多个瘤灶、低分化和有血管侵犯的原发性肝癌组织中 P2 7呈低表达 ;P2 7高表达的原发性肝癌患者较低表达者生存期明显延长 (P<0 .0 1)。结论　 P2 7基因在原发性肝癌组织中的表达有组织特异性 ;P2 7与原发性肝癌的浸润和转移有密切关系 ,可以作为原发性肝癌的预后判断指标之一

Expression and significance of P27 gene in primary hepatic carcinoma

Objective To study the expression of P 27 gene in the primary hepatic carcinoma (PHC)and the relation between P 27 and PHC prognosis.Methods Immunohistochemical staining was performed on 43 cases of PHC as well as 21 of cirrhosis and 16 of normal control specimens,used antibodies to P 27.Results P 27 mainly located in nucleolus,and all presented yellow grain.P 27 highly expressed in PHC group whereas rarely in normal and control groups.P 27 expression was significantly decreased in cases with mass diameter>5cm,poor differentiation,portal vascular invasion and multitumor(P<0.05).The survival analysis of P 27 was significantly different by following up 29 cases with hepatectomy.PHC with P 27 high expression showed better outcomes.Conclusions P 27 gene expression in PHC has particularity differing from other tumor inhibitor genes.P 27 gene expression is correlated with infiltration and metastasis in PHC.P 27 can be a novel prognostic marker for PHC.