甲状腺乳头状癌mir-221表达与BRAF突变的相关性研究

[目的]探讨甲状腺乳头状癌(PTC)中mir-221表达和BRAF基因状态的相关性和临床意义。[方法]采用RNA核酸印迹(Northern blot)法检测43例PTC癌组织及其癌旁正常组织中mir-221表达,直接测序法检测PTC癌组织中BRAF基因状态,分析mir-221表达和BRAF基因状态的相关性及其与临床病理特征的关系。[结果]mir-221在PTC癌组织中的表达显著高于配对正常组织(中位值:0.855vs0.549,P<0.001);mir-221高表达与临床分期(P=0.002)及淋巴结转移(P=0.042)有关。BRAF基因在PTC癌组织中的突变率为53.49%(23/43)。突变型BRAF的甲状腺癌患者组mir-221表达显著高于野生型BRAF患者组(P=0.024)。[结论]mir-221与BRAF基因在PTC的发生、发展过程中起重要作用,mir-221表达升高与BRAF基因突变有一定相关性。

Correlation between mir-221 Expression and BRAF Mutation in Papillary Thyroid Cancer

[Purpose] To investigate the correlation between mir-221 expression and BRAF mutation and theirs clinical significance in papillary thyroid cancer(PTC).[Methods] The mir-221 expression of specimens obtained from 43 cases with PTC and cancer adjacent tissues were examined by Northern blot.The status of BRAF of PTC was analyzed by direct DNA sequencing.The correlation between mir-221 expression and BRAF status with clinicopathologic features was analyzed.[Results] The expression of mir-221 in tumor tissues was significantly higher than that in normal thyroid tissues(medium 0.855 vs.0.549,P<0.001).The overexpression of mir-221 was correlated with TNM staging(P=0.002) and LN metastasis(P=0.042).BRAF mutation was found 53.49%(23/43) in all cases.The expression of mir-221 was significantly higher in BRAF mutation group than that in wild type group(P=0.024).[Conclusion] mir-221 and BRAF play an important role in carcinogenesis and progression of PTC.The overexpres of mir-221 may correlate with the BRAF mutation in PTC.