二氢石蒜碱对大鼠局灶性脑缺血/再灌注损伤的治疗作用

目的:观察二氢石蒜碱(dihydrolycorine,DL)对大鼠局部脑缺血/再灌注损伤的治疗作用和可能机制。方法:线栓法制备右侧大脑中动脉局灶性脑缺血模型,缺血1 h再灌注24 h。SD大鼠随机分为假手术组、模型组和DL低、中、高剂量组,于大鼠脑缺血后即刻及复灌后11 h及23 h DL组腹腔注射DL(10,20,40 mg.kg-1)治疗,假手术组和模型组则注射生理盐水对照。观察不同剂量DL对脑缺血再灌注大鼠神经功能状态、脑梗塞面积、脑水肿程度和脑组织髓过氧化物酶(myeloperoxidase,MPO)活性的影响。结果:脑缺血后给予DL可明显降低缺血侧脑梗死及脑水肿程度,使神经病学症状得到明显改善,降低缺血再灌注损伤后脑组织内MPO的活性(P<0.05)。结论:DL对脑缺血/再灌注损伤具有治疗作用,可能与其减轻脑缺血再灌注所致脑组织的炎症反应有关。

Therapeutic Effects of Dihydrolycorine on Focal Cerebral Ischemia-reperfusion Injury in Rats

Objective To investigate the therapeutic effects of dihydrolycorine(DL) on focal cerebral ischemia-reperfusion injury in rats.Methods The right middle cerebral artery ischemia models were established by ligating the internal carotid artery with a nylon suture for 1 h,following reperfusion for 24 h.SD rats were randomly divided into sham group,model group and different dosage of DL groups.DL(10,20,40 mg·kg-1) were respectively given at the beginning of cerebral ischemia immediately,11 h and 23 h after reperfusion through intraperitoneal injection.Normal saline were given in sham group and model group.The therapeutic effects of DL at different dosages were evaluated with neurological score,infract size,cerebral edema extent,myeloperoxidase(MPO) activity.Results The infarct size and cerebral edema extent were significantly decreased by giving DL after ischemia,the neurological function was significantly improved and the activity of MPO was significantly decreased correspondingly(P <0.05).Conclusion DL showed obvious therapeutic effects on cerebral ischemia reperfusion injury in rats and the effects may be related to the anti-inflammatory activity of DL.