强直性脊柱炎患者外周血T细胞亚群的变化及意义

目的探讨强直性脊柱炎（AS）患者外周血中T细胞亚群的变化及其意义。方法采用流式细胞仪（FCM）检测外周血淋巴细胞表面CD3、CD4、CD8及其胞质内细胞因子IFN-γ和IL-4的表达。结果与正常对照组相比，As患者外周血Th细胞（CD；CD4＋）百分率无显著差异，Tc细胞（CD；CD；）明显升高（P〈0．05）；Th1细胞（CD3^＋CD4^＋IFN-γ^＋）百分率明显升高，Th2细胞（CD3^＋CD4^＋IL4^＋）无显著性差异；Te1细胞（CD3^＋CD8^＋IFN-γ^＋）百分率明显降低，T02细胞（CD3^＋CD8^＋IL4^＋）无显著性差异。结论AS患者外周血T细胞Th1／Th2、Tcl／Tc2亚群比例失衡，呈Th1、Tc2优势型。

Changes of peripheral blood T cell subsets in ankylosing spondylitis patients and its significance

Objective To investigate the changes of peripheral blood T cell subsets in ankylosing spondylitis（AS） patients and its significance. Methods Flow eytometrie analysis was used to detect the expression of surface antigens CD3 , CD4 , CD8 and intracellular cytokines IFN-γ, IL-4 of T cells in the peripheral blood of patients with AS. Results Compared with normal controls, the percentage of Tc ceils（ CD3^＋ CD8^＋）in patients with AS increased significantly ,but the percentage of Th cells （ CD3^＋ CD4^＋） showed no significant changes. The percentage of Thl cells （ CD3^＋ CD4^＋ IFN-γ ） increased significantly,and the percentage of Th2 cells（ CD3^＋ CD4^＋ IL-4^＋ ） showed no significant changes. The percentage of Tel cells （CD3^＋ CD8^＋ IFN-γ^＋ ） decreased significantly,and the percentage of To2 ceils（ CD3^＋ CD8^＋ IL-4^＋ ） showed no significant changes. Conclusion There exists Thl/Th2 and Tcl/Tc2 T cell subsets disequilibrium in patients with AS ,which shows strong predominance of Thl and To2.