复合微量营养素对C57BL糖尿病小鼠Th1、Th2型细胞因子表达的调控作用

目的通过研究复合微量营养素(MC)对C57BL糖尿病小鼠Th1、Th2型细胞因子抗原表达的影响,探讨AM拮抗糖尿病的分子作用机制。方法应用小剂量多次注射STZ的方法(multiplelowdoseofstreptozotocin,MLDS)制备小鼠IDDM模型,经灌胃联合投予硒(Se)、维生素E(VE)、钒(V)、铬(Cr),应用流式细胞术观察表达Th1型细胞因子(TNFα、IFNγ)和Th2型细胞因子(IL4、IL10)抗原的淋巴细胞数百分比。结果IDDM小鼠外周血表达TNFα、IFNγ抗原的淋巴细胞数百分比明显升高(P<001),而外周血、脾组织表达IL10抗原的淋巴细胞数百分比明显降低(P<001);应用MC可显著降低IDDM小鼠外周血淋巴细胞TNFα的表达(P<001),显著升高外周血淋巴细胞IL10、脾淋巴细胞IL4的表达(P<001,P<005)。结论MC可通过下调Th1型细胞因子、上调Th2型细胞因子的基因表达,来调节IDDM时的免疫失衡,减轻胰岛自身免疫性损害,拮抗糖尿病的发生发展。

Regulatory effects of micronutrient complex on the expression of Th1 and Th2 cytokines in diabetic C57BL mice

OBJECTIVE: To investigate the regulatory effects of micronutrients complex (MC) on the expression of Th1 and Th2 cytokines in diabetic mice for exploring the molecular mechanisms of MC in treating for diabetes. METHODS: IDDM mice model was made by the injection of multiple low dose of streptozotocin (MLDS). The composition of Selenium (Se), Vitamin E (VE), Vanadium (V) and Chrome (Cr) was supplemented. The percentage of Th1 cytokines(TNF-alpha, IFN-gamma) and Th2 cytokines (IL-4, IL-10) positive lymphocytes were detected by flow cytometer (FCM). RESULTS: The expression of TNF-alpha and IFN-gamma of peripheral blood lymphocytes of MLDS group significantly increased (P < 0.01), while the IL-10 expression of blood and spleen lymphocytes of MLDS group obviously decreased (P < 0.01). Combined supplementation of MC markedly decreased blood lymphocytes TNF-alpha expression (P < 0.01) and increased blood lymphocytes IL-10 expression (P < 0.01) and spleen lymphocytes IL-4 expression (P < 0.05) of IDDM mice respectively. CONCLUSION: MC may prevent from the onset and development of IDDM by down-regulating Th1 cytokines genes expression and up-regulating Th2 cytokines genes expression of IDDM mice.