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| 人肝再生增强因子基因组DNA的克隆化与序列分析 | |
| 引用本文： | 成军,钟彦伟,刘妍,董菁,杨继珍,陈菊梅. 人肝再生增强因子基因组DNA的克隆化与序列分析[J]. 传染病信息, 1999, 0(2) |
| 作者姓名： | 成军 钟彦伟 刘妍 董菁 杨继珍 陈菊梅 |
| 作者单位： | 解放军传染病研究所基因治疗研究中心，解放军传染病研究所基因治疗研究中心，解放军传染病研究所基因治疗研究中心，解放军传染病研究所基因治疗研究中心，解放军传染病研究所基因治疗研究中心，解放军传染病研究所基因治疗研究中心 北京 100039，北京 100039，北京 100039，北京 100039，北京 100039，北京 100039 |
| 摘 要： | 目的克隆人肝再生增强因子(ALR)的基因组DNA,并进行序列分析。方法采用人肝再生增强因子cDNA及其编码序列,对基因的核苷酸序列数据库(GenBank)以BLAST为工具进行核苷酸序列同源性比较分析,寻找相应的ALR基因组DNA序列。结果从GenBank核苷酸序列数据库中寻找到人ALR基因组DNA全序列,由1813个核苷酸组成。人ALR基因组DNA序列由3段外显示子(exon)组成,分别为18nt,197nt和163nt。基因的编码产物由125个氨基酸残基组成,与小鼠ALR基因组DNA结构类似。基因组DNA定位于染色体的16p13.3位点上。结论克隆了人ALR基因组DNA全序列。 |
| 关 键 词： | 人肝再生增强因子 肝脏 基因克隆化 |
Cloning and Sequence Analysis of Human Genomic DNA of Augmenter of Liver Regeneration |
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| Cheng Jun,Zhong Yanwei,Liu Yan,et al.. Cloning and Sequence Analysis of Human Genomic DNA of Augmenter of Liver Regeneration[J]. Infectious Disease Information, 1999, 0(2) | |
| Authors: | Cheng Jun Zhong Yanwei Liu Yan et al. |
| Affiliation: | Cheng Jun,Zhong Yanwei,Liu Yan,et al. Gene Therapy Research Center,Institute of Infectious Diseases Beijing 100039 |
| Abstract: | Objective To clone the human genomic DNA of augmenter of liver regeneration (ALR) and specify the intron-exon structure. Methods Using human ALR cDNA sequence as a reference and BLAST path as a nucleotide homology search tool, GenBank has been searched for ALR homologous genomic DNA sequence. The intron-exon sequences were defined by the Breathnath-Chambon rule. Results The coding sequence of human ALR consists of 3 exons, which are 18nt, 19nt and 163nt. It is similar to murine ALR genomic DNA structure. The human ALR genomic DNA is 1813 nt long and codes a protein of 125 amino acid residues. Conclusion Human genomic DNA of ALR consists of 3 exons and 2 introns. |
| Keywords: | ALR Liver Cloning |
| 本文献已被 CNKI 等数据库收录！ | |