颈动脉内膜切除术后再狭窄动物模型的建立

目的：建立一种稳定的动脉内膜切除术后再狭窄动物模型。方法：日本大耳白兔30只。高脂饲料喂养2周进行手术，采用自身对照方法，右侧颈总动脉作为对照组，仅进行动脉游离。左侧颈总动脉为实验组，游离动脉并在动脉的中膜层内分离和切除内膜。术后继续给予高脂喂养2周。于术后4周、8周和12周取血和动脉标本。测定血脂含量，测量动脉内膜面积、中膜面积和中膜内膜面积比值。结果：27只动物存活，对照组无再狭窄发生，而实验组全部发生再狭窄，新生内膜形成是再狭窄的原因，且再狭窄程度随时间延长，程度加重，并在术后8周达到高峰。结论：颈总动脉内膜切除加高脂喂养，可建立稳定的再狭窄动物模型。

Restenosis Animal Model after Carotid Endarterectomy

Objective: To establish an animal model of the restenosis after carotid endarterectomy. Methods: Thirty Japanese white rabbits were operated after 2 weeks fat enriched feeding. The right common carotid arteries of rabbits were merely exposed as the control group. Carotid endarterectomy was performed on the left common carotid arteries in rabbits as the experimental group. Results: Twenty-seven rabbits remained alive no restenosis were observed in the control segments of arteries. Restenosis were observed in all the segments of arteries performed the carotid endarterectomy. Restenosis were mainly caused by the formation of neointima. The neointima area grew consistently in time and reached the peak at 8 weeks after the operation. Conclusion: It is a highly effective and steady animal model of restenosis after carotid endarterectomy and fat enriched feeding.