BrdU/NeuN双标记法研究复方刺蒺藜苷抗抑郁的机制

目的：观察复方刺蒺藜苷对慢性应激抑郁大鼠海马神经元发生的影响,并探讨其机理。方法：采用慢性应激加孤养建立大鼠抑郁模型,通过敞箱实验等观察抑郁模型大鼠行为学改变,BrdU/NeuN标记海马齿状回阳性细胞反映神经元发生,分化。结果：慢性应激抑郁大鼠体重增长缓慢,蔗糖溶液消耗量减少,敞箱实验中水平穿越格数、竖立次数、理毛时间减少,中央格停留时间、粪便粒数均增加,与正常组比较具有显著性差异（P〈0.05或P〈0.01）。复方刺蒺藜苷和氟西汀可改善大鼠行为学变化,增加BrdU/NeuN阳性细胞数,与模型组比较具有显著性差异（P〈0.05或P〈0.01）。结论：复方刺蒺藜苷能明显改善抑郁大鼠的各项行为学指标,促进海马神经元发生,具有抗抑郁作用。

Antidepressant Mechanism of Tribuloside on Chronic Stress Depression Rats By NeuN/BrdU Double Label Method

Objective ：To observe the effect of tribuloside on hippocampus of chronic stress depression rats. Methods：All rats were singly housed and exposed to an unpredicted sequence of mild stressor. The behavior of rats was detected by open-field test. And neuron genesis and differentiation in dentate gyrus was observed by BrdU/NeuN. Results：Compared with control group, the weight of rats increased slowly, with little consumption of cane sugar. Squares crossed, rear times and grooming in open-field test reduced, while time of staying in central square and defecation increased significantly （P〈 0.01 ,P〈0.05）. Fluoxetine or compound tribuloside significantly improved behavioral disorder and BrdU/NeuN immunoreactive cells （P〈0.05）. Conclusion ：Tribuloside can improve behavior and BrdU/NeuN immunoreactive cells of depression model rats and has antidepressant function.