喉癌组织内微血管密度的测定及其临床意义

目的 :探讨喉癌组织内微血管的形成情况及其临床意义。方法 :采用第 因子相关抗原免疫组化法定量观察 38例喉癌组织内微血管的形态和分布并计算微血管密度 (MVD)。结果 :1喉癌组织内血管形态不规则 ,血管分布具有异质性 ,在癌巢实质内存在几无血管的“空白区”。 2癌组织中的 MVD显著高于癌周正常组织 (P <0 .0 1)。 3颈部淋巴结转移组喉癌组织 MVD高于非转移组 (P <0 .0 1) ; 、 期喉癌组织 MVD高于 、 期 (P <0 .0 1)。 4声门上型喉癌组织 MVD与声门型相比较差异无显著性意义 (P >0 .0 5 )。 5喉癌组织内 MVD在高分化、中分化、低分化三组中分别为 (2 4.70± 6 .36 )、(2 8.6 4± 11.5 4)、(2 9.0 7± 7.84)个 / HPF。其差异无显著性意义。结论 :1喉癌组织内存在血管增生现象 ,增生血管主要位于肿瘤间质 ,分布具有异质性。 2喉癌组织内MVD与喉癌分型及组织学分级无明显关系。 3肿瘤血管的形成有利于肿瘤的侵袭转移 ,喉癌组织 MVD的测定可望作为评估预后的又一重要指标。

Measurement of microvessel density in laryngeal carcinoma and its clinical significance

OBJECTIVE: To determine the microvessel density (MVD) in laryngeal carcinoma and its clinical significance. METHOD: Thirty-eight tumor specimens were selected from laryngeal cancer patients from January, 1994 to March, 1996. Histological sections of the tumors were stained immunohistochemically for factor VIII. Using light microscopy, we counted microvessels per 400 x field in the most active areas of tumor angiogenesis. RESULT: 1. The tumor blood vessels, composed of only one layer of endothelium were mainly distributed heterogeneously in the interstitial tissue of laryngeal carcinoma with irregular lumen, poorly developed structure. 2. The MVD in the cancer tissues were statistically higher than that in peritumoral tissues (P < 0.01). 3. The MVD in the cancer tissues in group of patients with metastasis to cervical lymphonodes were statistically higher than in group without metastasis (P < 0.01), the MVD in the cancer tissues in group of advanced cases (III, IV stages) were statistically higher than that in group of early cases (I, II stages, P < 0.01). 4. There was no statistically difference in MVD in the cancer tissue between supraglottic and glottic laryngeal carcinoma patients (P > 0.05). 5. There was no statistically difference in MVD in the cancer tissue among the G1, G2 and G3 group (P > 0.05). CONCLUSION: The laryngeal cancer blood vessels have some characteristics that don't appear in normal vessels. It is suggested that tumor angiogenesis can promote tumor growth and metastasis and MVD may be a new prognostic indicator of laryngeal carcinoma.