| X射线全身照射对小鼠骨髓细胞周期的影响 |
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| 引用本文： | 鞠桂芝,马淑梅,傅士波,刘树铮.X射线全身照射对小鼠骨髓细胞周期的影响[J].中国预防医学杂志,2002,3(2):87-88. |
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| 作者姓名： | 鞠桂芝 马淑梅 傅士波 刘树铮 |
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| 作者单位： | 吉林大学白求恩医学部卫生部放射生物学重点实验室长春,130021 |
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| 基金项目： | 国家自然科学基金资助项目(编号:39770193) |
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| 摘 要： | 目的 研究不同剂量X射线全身照射对小鼠骨髓细胞周期的影响。方法 采用PI标记及流式细胞术（FCM）检测细胞周期的变化。结果 发现，1.0-6.0GyX射线线全身照射后12h，小鼠骨髓细胞G1及G2期明显增高，而S期明显减少；同时发现，0.05-0.2Gy低剂量X射线全身照射后72h,G1期骨髓细胞明显减少。而S期细胞明显增高。结论 0.5Gy以上剂量X射线全身照射可诱导小鼠骨髓细胞G1及G2期阻滞，使骨髓细胞增殖明显受抑；而0.2Gy以下低剂量照射则可刺激骨髓细胞增殖。
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| 关 键 词： | X射线 全身照射 细胞周期 电离辐射 骨髓 PI标记 流式细胞术 FCM 动物实验 |
| 修稿时间： | 2001年9月3日 |
Effect of X-irradiation on Mice Bone Marrow Cell Cycle |
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| JUGui-zhi,MAShu-mei,FU Shi-bo,et al..Effect of X-irradiation on Mice Bone Marrow Cell Cycle[J].China Preventive Medicine,2002,3(2):87-88. |
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| Authors: | JUGui-zhi MAShu-mei FU Shi-bo |
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| Institution: | JUGui-zhi,MAShu-mei,FU Shi-bo,et al. MH of Radiobiolo-gy Research Unit,Medical Center in Memorial of Norman Bethune of Jilin University,Changchun 130021,China |
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| Abstract: | To investigate the effect of whole body X - irradiation with different doses on mice bone marrow cell cycle.Methods Cell cycle was analyzed by flow cytometry (FCM) following staining of cells with propidium iodide (PI) . Results It was found that G1 and G2 phases of bone marrow cells increased significantly at 12h after whole body irradiation (WBI) with the doses of 1.0, 2.0, 4.0 and 6.0 Gy, whereas S phase cells were decreased at the same times.However, S phase was increased significantly at 72h following 0.05, 0.075, 0.1 and 0.2 Gy low dose X- irradiation.Conclusion These results suggest that Gl and G2 arrest could be induced by X - irradiation with the doses above 0.5Gy.S phase could be stimulated by low dose X - rays below 0.2Gy. |
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| Keywords: | Radiation ionizing Cell cycle Bone marrow |
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