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XTT assay for evaluating the effect of alcohols, hydrogen peroxide and benzalkonium chloride on biofilm formation of Staphylococcus epidermidis |
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| Authors: | Chaieb Kamel Zmantar Tarek Souiden Yosra Mahdouani Kacem Bakhrouf Amina |
| Affiliation: | Laboratoire d'Analyses, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne 5000 Monastir, Tunisia. chaieb_mo@yahoo.fr |
| Abstract: | To analyze the degree of biofilm formation on three ica-positives Staphylococcus epidermidis as a function of biocides, the medium was supplemented with increasing concentrations of isopropanol, ethanol, and methanol at 0, 1, 4, 6, 8, 10, 12 and 14% (v/v), hydrogen peroxide (0, 0.125, 0.25, 0.5, 1, 2, 3, 4 and 5% v/v) and benzalkonium chloride (0, 0.125, 0.25, 0.5, 1, 2, 3, 4 and 6 μg ml−1). |
| Keywords: | Staphylococcus epidermidis Alcohols Hydrogen peroxide Benzalkonium chloride Biofilm XTT |
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