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| 构建IL-2优化的免疫球蛋白抗淋巴瘤基因疫苗 | |
| 引用本文： | 刘辉,常乃柏,顾惜春,朱平. 构建IL-2优化的免疫球蛋白抗淋巴瘤基因疫苗[J]. 中国实验血液学杂志, 2006, 14(6): 1160-1162 |
| 作者姓名： | 刘辉 常乃柏 顾惜春 朱平 |
| 作者单位： | 1. 卫生部北京医院血液科,北京100730 2. 北京大学第一医院血液内科,北京100034 |
| 摘 要： | 本研究构建白介素2（IL-2）基因与免疫球蛋白重链可变区（IgHV）基因共表达的基因疫苗。从淋巴瘤患者外周血获得免疫球蛋白基因IgHV片段，将其与IL-2基因连接后形成的融合基因克隆到真核表达载体中。表达载体通过脂质体转染COS细胞，用ELISA法检测IL-2的表达。结果显示：本研究成功构建了IgHV基因与IL-2基因的融合基因表达载体。在IgHV-IL-2／pcDNA3．0中IL-2可以获得正确表达。结论：本研究构建的IL-2和IgHV的融合表达载体可以在COS细胞中分泌表达IL-2。 |
| 关 键 词： | 基因疫苗 白介素2 淋巴瘤 |
| 文章编号： | 1009-2137（2006）06-1160-03 |
| 收稿时间： | 2005-12-01 |
| 修稿时间： | 2006-08-24 |
Construction of Fusion Gene Between IgGHV and IL-2 as IgHV Nucleic Acid Vaccine Against Lymphoma |
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| LIU Hui,CHANG Nai-Bai,GU Xi-Chun,ZHU Ping. Construction of Fusion Gene Between IgGHV and IL-2 as IgHV Nucleic Acid Vaccine Against Lymphoma[J]. Journal of experimental hematology, 2006, 14(6): 1160-1162 | |
| Authors: | LIU Hui CHANG Nai-Bai GU Xi-Chun ZHU Ping |
| Affiliation: | Department of Hematology, Beijing Hospital, Ministry of Health, Beijing 100730, China. |
| Abstract: | The purpose of this study was to construct the IgHV and IL-2 coexpressed vector. The IgHV gene fragments were obtained from the peripheral blood of patients with lymphoma, and were cloned into eukaryotic expression vector. Meanwhile, the gene fragments of IgHV linked with gene of IL-2 were inserted into pcDNA3.0 to form a fusion gene of IgHV-IL-2. Then fusion genes were transfected into COS cells by Lipofectin and the expression of IL-2 was detected by ELISA. The results showed that the IgHV/pcDNA3.0 expression vector was successfully constructed. The 3' end of IgHV was linked to IL-2 gene, and IL-2 could be correctly expressed. In conclusion, the expression vector of IgHV-IL-2 can express IL-2 correctly in COS cells. |
| Keywords: | gene vaccine IL-2 Lymphoma |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |